Evidence map›Paper›PMID 24598548›Full record

ArticleScientific reports2014

Genomics in premature infants: a non-invasive strategy to obtain high-quality DNA.

Mariam Said, Clint Cappiello, Joseph M Devaney, Daniele Podini, Alana L Beres, Stanislav Vukmanovic, Khodayar Rais-Bahrami, Naomi C Luban, Anthony D Sandler, Zohreh Tatari-Calderone

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Mariam Said1] Division of Neonatology, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Clint CappielloSheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA.
Joseph M Devaney1] Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Daniele PodiniDepartment of Forensic Science, The George Washington University, Washington, DC; USA.
Alana L BeresSheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA.
Stanislav Vukmanovic1] Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Khodayar Rais-Bahrami1] Division of Neonatology, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Naomi C Luban1] Division of Laboratory Medicine, Children's National Health System, Washington, DC; USA [2] Division of Hematology and Oncology, Children's National Health System, Washington, DC; USA [3] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Anthony D Sandler1] Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of General Surgery, Children's National Health System, Washington, DC; USA [3] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Zohreh Tatari-Calderone1] Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, 111 Michigan Avenue NW, Washington, DC; USA [2] Department of Pediatrics, George Washington University School of Medicine, Washington, DC; USA.
Children's National · USGeorge Washington University · US

Funding

CTSA INFRASTRUCTURE FOR PEDIATRIC RESEARCHUL1RR031988 · NCRR · CHILDREN'S RESEARCH INSTITUTE · PI JOSEPH, JILL G · 2010 to 2011
$8.2M
NCRR NIH HHS UL1RR031988
6 · The paper itself

Abstract

We used a cost-effective, non-invasive method to obtain high-quality DNA from buccal epithelial-cells (BEC) of premature infants for genomic analysis. DNAs from BEC were obtained from premature infants with gestational age ≤ 36 weeks. Short terminal repeats (STRs) were performed simultaneously on DNA obtained from the buccal swabs and blood from the same patient. The STR profiles demonstrated that the samples originated from the same individual and exclude any contamination by external DNAs. Whole exome sequencing was performed on DNAs obtained from BEC on premature infants with and without necrotizing enterocolitis, and successfully provided a total number of reads and variants corroborating with those obtained from healthy blood donors. We provide a proof of concept that BEC is a reliable and preferable source of DNA for high-throughput sequencing in premature infants.

Indexed as

GenomicsInfant, PrematureEpithelial CellsExomeGenetic LociHigh-Throughput Nucleotide SequencingHumansInfant, NewbornMicrosatellite RepeatsMouth Mucosa

Identifiers

PMID24598548
PMCPMC3944721
OpenAlexW1992730735

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.