ArticleAmerican journal of physiology. Cell physiology2014
Collagen content does not alter the passive mechanical properties of fibrotic skeletal muscle in mdx mice.
Article in American journal of physiology. Cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 128 citations in OpenAlex.
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- Juvenile vs. adult skeletal muscle transplants in the treatment of volumetric muscle loss injury.Stem cell research & therapy · 2025Article
- Collagenase treatment does not impair fiber contractile function in muscle biopsies from children with cerebral palsy.Physiological reports · 2025Article
- Intramuscular adipose tissue restricts functional muscle recovery.Cell reports · 2025Article
- Biomechanical study of myogenic factors in the contracture period of traumatic elbow joint.Journal of orthopaedics · 2025Article
- Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage.Cell and tissue research · 2025Article
- Dysregulated ATX-LPA and YAP/TAZ signaling in dystrophic SgcdSkeletal muscle · 2025Article
- Evaluation of radiation therapy on grafted and non-grafted defects: an experimental rat model.Journal of applied oral science : revista FOB · 2025Article
- Strain-dependent dynamic re-alignment of collagen fibers in skeletal muscle extracellular matrix.Acta biomaterialia · 2024Article
- Collagen architecture and biomechanics of gracilis and adductor longus muscles from children with cerebral palsy.The Journal of physiology · 2024Article
- Effect of nonsteroidal anti-inflammatory drugs on pelvic floor muscle regeneration in a preclinical birth injury rat model.American journal of obstetrics and gynecology · 2024Article
- It's more than the amount that counts: implications of collagen organization on passive muscle tissue properties revealed with micromechanical models and experiments.Journal of the Royal Society, Interface · 2024Article
- GDF8 inhibition enhances musculoskeletal recovery and mitigates posttraumatic osteoarthritis following joint injury.Science advances · 2023Article
- Effects of different doses of ionizing radiation on alveolar bone repair in post-extraction tooth socket: an experimental study in rats.Clinical oral investigations · 2023Article
- Alignment, cross linking, and beyond: a collagen architect's guide to the skeletal muscle extracellular matrix.American journal of physiology. Cell physiology · 2023Review
20 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Many skeletal muscle diseases are associated with progressive fibrosis leading to impaired muscle function. Collagen within the extracellular matrix is the primary structural protein providing a mechanical scaffold for cells within tissues. During fibrosis collagen not only increases in amount but also undergoes posttranslational changes that alter its organization that is thought to contribute to tissue stiffness. Little, however, is known about collagen organization in fibrotic muscle and its consequences for function. To investigate the relationship between collagen content and organization with muscle mechanical properties, we studied mdx mice, a model for Duchenne muscular dystrophy (DMD) that undergoes skeletal muscle fibrosis, and age-matched control mice. We determined collagen content both histologically, with picosirius red staining, and biochemically, with hydroxyproline quantification. Collagen content increased in the mdx soleus and diaphragm muscles, which was exacerbated by age in the diaphragm. Collagen packing density, a parameter of collagen organization, was determined using circularly polarized light microscopy of picosirius red-stained sections. Extensor digitorum longus (EDL) and soleus muscle had proportionally less dense collagen in mdx muscle, while the diaphragm did not change packing density. The mdx muscles had compromised strength as expected, yet only the EDL had a significantly increased elastic stiffness. The EDL and diaphragm had increased dynamic stiffness and a change in relative viscosity. Unexpectedly, passive stiffness did not correlate with collagen content and only weakly correlated with collagen organization. We conclude that muscle fibrosis does not lead to increased passive stiffness and that collagen content is not predictive of muscle stiffness.
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