ArticleNucleic acids research2014
Bacterial promoter repression by DNA looping without protein-protein binding competition.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Defining the DNA Binding Specificity of GRHL2.bioRxiv : the preprint server for biology · 2026Article
- Molecular insights into the fine-tuning of pH-dependent ArsR-mediated regulation of the SabA adhesin in Helicobacter pylori.Nucleic acids research · 2024Article
- Single-molecule visualization of twin-supercoiled domains generated during transcription.Nucleic acids research · 2024Article
- High-Resolution Characterization of DNA/Protein Complexes in Living Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis.Nucleic acids research · 2022Article
- Relative, local and global dimension in complex networks.Nature communications · 2022Article
- Designed architectural proteins that tune DNA looping in bacteria.Nucleic acids research · 2021Article
- The loopometer: a quantitative in vivo assay for DNA-looping proteins.Nucleic acids research · 2021Article
- Characterization of Gene Repression by Designed Transcription Activator-like Effector Dimer Proteins.Biophysical journal · 2020Article
- Bacterial gene control by DNA looping using engineered dimeric transcription activator like effector (TALE) proteins.Nucleic acids research · 2018Article
- Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.Biophysical reviews · 2016Review
- Supercoiling biases the formation of loops involved in gene regulation.Biophysical reviews · 2016Review
- Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.Biophysical reviews · 2016Review
- Metal-responsive promoter DNA compaction by the ferric uptake regulator.Nature communications · 2016Article
- Direct observation of a 91 bp LacI-mediated, negatively supercoiled DNA loop by atomic force microscope.FEBS letters · 2016Article
- Supercoiling Effects on Short-Range DNA Looping in E. coli.PloS one · 2016Article
- Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins.The Journal of biological chemistry · 2015Review
- High-resolution mapping of architectural DNA binding protein facilitation of a DNA repression loop in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Escherichia coli lactose operon provides a paradigm for understanding gene control by DNA looping where the lac repressor (LacI) protein competes with RNA polymerase for DNA binding. Not all promoter loops involve direct competition between repressor and RNA polymerase. This raises the possibility that positioning a promoter within a tightly constrained DNA loop is repressive per se, an idea that has previously only been considered in vitro. Here, we engineer living E. coli bacteria to measure repression due to promoter positioning within such a tightly constrained DNA loop in the absence of protein-protein binding competition. We show that promoters held within such DNA loops are repressed ∼100-fold, with up to an additional ∼10-fold repression (∼1000-fold total) dependent on topological positioning of the promoter on the inner or outer face of the DNA loop. Chromatin immunoprecipitation data suggest that repression involves inhibition of both RNA polymerase initiation and elongation. These in vivo results show that gene repression can result from tightly looping promoter DNA even in the absence of direct competition between repressor and RNA polymerase binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.