ArticleCurrent chemical genomics and translational medicine2014
Live multicellular tumor spheroid models for high-content imaging and screening in cancer drug discovery.
Article in Current chemical genomics and translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Precision medicine research progress based on colorectal cancer organoids.Discover oncology · 2025Review
- Review
- Advancing cancer research through 3D cell culture models.EXCLI journal · 2025Review
- Next-Generation Adjuvants: Applying Engineering Methods to Create and Evaluate Novel Immunological Responses.Pharmaceutics · 2023Article
- Assay establishment and validation of a high-throughput organoid-based drug screening platform.Stem cell research & therapy · 2022Article
- The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death.Pharmaceutics · 2022Article
- Drug Design Targeting T-Cell Factor-Driven Epithelial-Mesenchymal Transition as a Therapeutic Strategy for Colorectal Cancer.Journal of medicinal chemistry · 2019Article
- A Human iPSC-derived 3D platform using primary brain cancer cells to study drug development and personalized medicine.Scientific reports · 2019Article
- High-content imaging assays on a miniaturized 3D cell culture platform.Toxicology in vitro : an international journal published in association with BIBRA · 2018Article
- Pharmacological targeting of GLI1 inhibits proliferation, tumor emboli formation and in vivo tumor growth of inflammatory breast cancer cells.Cancer letters · 2017Article
- Article
- Cross talk between progesterone receptors and retinoic acid receptors in regulation of cytokeratin 5-positive breast cancer cells.Oncogene · 2017Article
- High-throughput imaging: Focusing in on drug discovery in 3D.Methods (San Diego, Calif.) · 2016Review
- Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids.Journal of visualized experiments : JoVE · 2015Article
- Review
- High-throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models.Expert opinion on drug discovery · 2015Review
- Article
- Steroid hormones, steroid receptors, and breast cancer stem cells.Journal of mammary gland biology and neoplasia · 2015Review
- Article
- Editorial: high content screening for lead identification and optimization.Current chemical genomics and translational medicine · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The multi cellular tumor spheroid (MCTS) model has been used for decades with proven superiority over monolayer cell culture models at recapitulating in vivo tumor growth. Yet its use in high-throughput drug discovery has been limited, particularly with image based screening, due to practical and technical hurdles. Here we report a significant advance in utilizing live MCTS models for high-content image based drug discovery. Using a validated GFP reporter (CK5Pro-GFP) of luminal breast cancer stem cells (CSC), we developed an algorithm to quantify changes in CK5Pro-GFP expression levels for individual Z-stack planes (local) or as maximal projections of the summed Z-stacks (global) of MCTS. From these image sets, we can quantify the cross-sectional area of GFP positive cells, the fluorescence intensity of the GFP positive cells, and the percent of spheroid cross-sectional area that expresses CK5Pro-GFP.We demonstrate that acquiring data in this manner can be done in real time and is statistically robust (Z'=0.85) for use in primary high-content screening cancer drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.