ArticleThe Journal of biological chemistry2014
Phosphorylation regulates the p31Comet-mitotic arrest-deficient 2 (Mad2) interaction to promote spindle assembly checkpoint (SAC) activity.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- An epigenome-wide association meta-analysis of prenatal maternal stress in neonates: A model approach for replication.Epigenetics · 2016Pooled it
- Linear ubiquitination of p31Cell & bioscience · 2025Article
- p31International journal of molecular sciences · 2025Article
- Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023Review
- Kinetochore-catalyzed MCC formation: A structural perspective.IUBMB life · 2023Article
- Inhibition of MAD2L1 Mediates Pulmonary Fibrosis through Impairment of Mitochondrial Function and Induction of Cell Senescence.Canadian respiratory journal · 2022Article
- p31Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- p31Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The conserved AAA-ATPase PCH-2Molecular biology of the cell · 2020Article
- Review
- Role of Polo-like kinase 1 in the regulation of the action of p31Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic Signaling.PLoS pathogens · 2015Article
- The multifaceted roles of the HORMA domain in cellular signaling.The Journal of cell biology · 2015Review
- TRIP13PCH-2 promotes Mad2 localization to unattached kinetochores in the spindle checkpoint response.The Journal of cell biology · 2015Article
- Article
- Article
- Phosphorylation propels p31(comet) for mitotic exit.Cell cycle (Georgetown, Tex.) · 2015Article
- Phosphorylation of Xenopus p31(comet) potentiates mitotic checkpoint exit.Cell cycle (Georgetown, Tex.) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The spindle assembly checkpoint (SAC) ensures the faithful segregation of the genome during mitosis by ensuring that sister chromosomes form bipolar attachments with microtubules of the mitotic spindle. p31(Comet) is an antagonist of the SAC effector Mad2 and promotes silencing of the SAC and mitotic progression. However, p31(Comet) interacts with Mad2 throughout the cell cycle. We show that p31(Comet) binds Mad2 solely in an inhibitory manner. We demonstrate that attenuating the affinity of p31(Comet) for Mad2 by phosphorylation promotes SAC activity in mitosis. Specifically, phosphorylation of Ser-102 weakens p31(Comet)-Mad2 binding and enhances p31(Comet)-mediated bypass of the SAC. Our results provide the first evidence for regulation of p31(Comet) and demonstrate a previously unknown event controlling SAC activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.