ArticleLasers in medical science2014
Laser-induced osteoblast proliferation is mediated by ROS production.
Article in Lasers in medical science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 4 of them syntheses that pooled it.
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The trial behind it
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Who cites it
51 citing papers in PubMed, 4 syntheses or guidelines pooled it, 122 citations in OpenAlex.
- Radiographical impact of photobiomodulation therapy on bone regeneration in clinical studies: a systematic review.Lasers in medical science · 2025Pooled it
- Response of osteoblastic cells to low-level laser treatment: a systematic review.Lasers in medical science · 2022Pooled it
- Bioenergetics of photobiomodulated osteoblast mitochondrial cells derived from human pulp stem cells: systematic review.Lasers in medical science · 2022Pooled it
- Review of light parameters and photobiomodulation efficacy: dive into complexity.Journal of biomedical optics · 2018Pooled it
- Adjuvant therapy with a 980-nm diode laser in root canal retreatment: randomized clinical trial with 1-year follow-up.Lasers in medical science · 2023Trial
- Effects of photobiomodulation on human dental pulp stem cells treated with a bone xenograft: An in vitro study.Lasers in medical science · 2026Article
- In vitro investigation of the effect of laser on platelet-rich fibrins.BMC oral health · 2026Article
- A Biomimetic Approach to Diode Laser Use in Endodontic Treatment of Immature Teeth: Thermal, Structural, and Biological Analysis.Biomimetics (Basel, Switzerland) · 2025Article
- Biocompatibility and photoinactivation evaluation of zinc hydroxide chloride nanosheets against Streptococcus mutans.BMC microbiology · 2025Article
- Combination of Different Laser Wavelengths as a Treatment Strategy for Maxillary Osteonecrosis: A Case Report.Journal of lasers in medical sciences · 2025Article
- Effect of nutritional stress and photobiomodulation protocol on in vitro viability and proliferation of murine preosteoblast cells.Lasers in medical science · 2024Article
- A novel investigational preclinical model to assess fluence rate for dental oral craniofacial tissues.Photodiagnosis and photodynamic therapy · 2024Article
- Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment.Lasers in medical science · 2024Article
- Deciphering the Mysterious Relationship between the Cross-Pathogenetic Mechanisms of Neurodegenerative and Oncological Diseases.International journal of molecular sciences · 2023Review
- Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway.Scientific reports · 2023Article
- Photobiomodulation at molecular, cellular, and systemic levels.Lasers in medical science · 2023Review
- The effect of low-level laser irradiation on the proliferation, osteogenesis, inflammatory reaction, and oxidative stress of human periodontal ligament stem cells under inflammatory conditions.Lasers in medical science · 2022Article
- Review
- Effect of Simvastatin and Low-Level Laser Therapy on Sutural Bone Formation After Expansion in Rats: Biomechanical, Computed Tomography and Immunohistochemical Assessment.Journal of lasers in medical sciences · 2022Article
- Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis.Stem cell research & therapy · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low-level laser therapy (LLLT) is widely used in regenerative medicine and in dental therapy by virtue of its beneficial effects in a plethora of pathological conditions. In this study, the effect of a 980 nm diode laser on pre-osteoblasts proliferation has been evaluated, along with reactive oxygen species (ROS) production. We hypothesized that ROS were a key factor in LLLT-induced pre-osteoblasts proliferation, as it is known that ROS can induce the activation of many biological pathways, leading to cell proliferation, differentiation or apoptosis. Murine pre-osteoblasts MC3T3 cells were irradiated with different energy outputs (1-50 J) in the absence or presence of the antioxidant N-Acetyl-L-cysteine (NAC). Laser treatment, in the absence of NAC, was able to induce a fluence-dependent statistically significant increase in ROS generation, while the presence of NAC strongly inhibited it. Cell proliferation, measured after laser stimulation, was significantly increased both at low and higher energy, with a peak at 10 J in the absence of the antioxidant. On the contrary, in the presence of NAC, laser irradiation was not able to induce any cell proliferation, suggesting a crucial role of ROS in this laser-induced cell effect. These results suggest that LLLT may be a useful tool for bone regeneration therapy and an effective range of fluences to be used is indicated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.