ArticleProtoplasma2014
Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat.
Article in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Pooled it
- The Effect of Sethoxydim Herbicide on the Physiological Parameters, Photosynthetic Enzymes and Antioxidant System in Foxtail Millet.Plants (Basel, Switzerland) · 2026Article
- Enhanced drought tolerance and photosynthetic efficiency inFrontiers in plant science · 2024Article
- Regulation of root growth and elongation in wheat.Frontiers in plant science · 2024Review
- Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency.Plants (Basel, Switzerland) · 2023Review
- Effects of exogenous Strigolactone on the physiological and ecological characteristics of Pennisetum purpureum Schum. Seedlings under drought stress.BMC plant biology · 2022Article
- Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.International journal of molecular sciences · 2022Review
- Transgenic expression of rice OsPHR2 increases phosphorus uptake and yield in wheat.Protoplasma · 2022Article
- Multi-Approach Analysis Reveals Pathways of Cold Tolerance Divergence inFrontiers in plant science · 2022Article
- Differences in Physiological Responses of Two Oat (Plants (Basel, Switzerland) · 2020Article
- Recent developments and applications of genetic transformation and genome editing technologies in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- The Pyruvate-Phosphate Dikinase (CFrontiers in plant science · 2020Article
- Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations.International journal of molecular sciences · 2019Review
- Proteomics Analysis to Identify Proteins and Pathways Associated with the Novel Lesion Mimic Mutant E40 in Rice Using iTRAQ-Based Strategy.International journal of molecular sciences · 2019Article
- Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing.BioMed research international · 2019Review
- Physiological characteristics and metabolomics of transgenic wheat containing the maize CProtoplasma · 2017Article
- Functions of OsDof25 in regulation of OsC4PPDK.Plant molecular biology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using particle bombardment transformation, we introduced maize pepc cDNA encoding phosphoenolpyruvate carboxylase (PEPC) and ppdk cDNA encoding pyruvate orthophosphate dikinase (PPDK) into the C3 crop wheat to generate transgenic wheat lines carrying cDNA of pepc (PC lines), ppdk (PK lines) or both (PKC lines). The integration, transcription, and expression of the foreign genes were confirmed by Southern blot, Real-time quantitative reverse transcription PCR (Q-RT-PCR), and Western blot analysis. Q-RT-PCR results indicated that the average relative expression levels of pepc and ppdk in the PKC lines reached 10 and 4.6, respectively, compared to their expressions in untransformed plants (set to 1). The enzyme activities of PEPC and PPDK in the PKC lines were 4.3- and 2.1-fold higher, respectively, than in the untransformed control. The maximum daily net photosynthetic rates of the PKC, PC, and PK lines were enhanced by 26.4, 13.3, and 4.5%, respectively, whereas the diurnal accumulations of photosynthesis were 21.3, 13.9, and 6.9%, respectively, higher than in the control. The Fv/Fm of the transgenic plants decreased less than in the control under high temperature and high light conditions (2 weeks after anthesis), suggesting that the transgenic wheat transports more absorbed light energy into a photochemical reaction. The exogenous maize C4-specific pepc gene was more effective than ppdk at improving the photosynthetic performance and yield characteristics of transgenic wheat, while the two genes showed a synergistic effect when they were transformed into the same genetic background, because the PKC lines exhibited improved photosynthetic and physiological traits.
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