ArticleInvestigative ophthalmology & visual science2014
Pilocarpine-induced dilation of Schlemm's canal and prevention of lumen collapse at elevated intraocular pressures in living mice visualized by OCT.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
- A Small Molecule Inhibitor of VE-PTP Activates Tie2 in Schlemm's Canal Increasing Outflow Facility and Reducing Intraocular Pressure.Investigative ophthalmology & visual science · 2020Trial
- Comparing the trabecular outflow by the response to topical pilocarpine in patients with and without glaucoma filtering surgery.Japanese journal of ophthalmology · 2020Trial
- Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction.iScience · 2026Article
- Machine-Learning-Based Automated Schlemm's Canal Volumetric Segmentation for Optical Coherence Tomography.Chemical & biomedical imaging · 2026Article
- Changes of Schlemm's Canal and Trabecular Meshwork Dimensions from Digital Ocular Massage in High Myopia: A Pilot Study.Ophthalmic research · 2026Article
- Evaluating Pilocarpine 1% Eye Drops in Presbyopia: A Triple-Blinded Randomized Trial.Journal of ophthalmology · 2026Article
- Robotic Optical Coherence Tomography With Expanded Three-Dimensional Field-of-View Using Point-Cloud-Based Volumetric Montaging.IEEE transactions on medical imaging · 2025Article
- Endothelial cell-specific postnatal deletion of Nos3 preserves intraocular pressure homeostasis via macrophage recruitment and NOS2 upregulation.The Journal of clinical investigation · 2025Article
- Robotic Visible-Light Optical Coherence Tomography Visualizes Segmental Schlemm's Canal Anatomy and Segmental Pilocarpine Response.Investigative ophthalmology & visual science · 2025Article
- The Change of Ciliary Muscle-Trabecular Meshwork-Schlemm Canal Complex after Phacoemulsification using Swept-Source-Optical Coherence Tomography.Ophthalmic research · 2025Article
- Low Intraocular Pressure Induces Fibrotic Changes in the Trabecular Meshwork and Schlemm's Canal of Sprague Dawley Rats.Translational vision science & technology · 2024Article
- Robotic Visible-Light Optical Coherence Tomography Visualizes Segmental Schlemm's Canal Anatomy and Segmental Pilocarpine Response.bioRxiv : the preprint server for biology · 2024Article
- A Histomorphometric and Computational Investigation of the Stabilizing Role of Pectinate Ligaments in the Aqueous Outflow Pathway.Journal of biomechanical engineering · 2024Article
- Aging and intraocular pressure homeostasis in mice.Aging cell · 2024Article
- A Histomorphometric and Computational Investigation of the Stabilizing Role of Pectinate Ligaments in the Aqueous Outflow Pathway.bioRxiv : the preprint server for biology · 2024Article
- Pressure Clamping During Ocular Perfusions Drives Nitric Oxide-Mediated Washout.Investigative ophthalmology & visual science · 2023Article
- Recent advances in optical coherence tomography for anterior segment imaging in small animals and their clinical implications.The ocular surface · 2022Review
- Drug-Loaded Polymeric Particulated Systems for Ophthalmic Drugs Release.Molecules (Basel, Switzerland) · 2022Article
- Measurement of postmortem outflow facility using iPerfusion.Experimental eye research · 2022Article
- Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.Investigative ophthalmology & visual science · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
purposeThe goal was to assess effects of IOP and pilocarpine-induced ciliary muscle contraction on conventional outflow pathway tissues in living anesthetized mice.
methodsIntraocular pressure was controlled by intracameral cannulation of mouse eyes while imaging using spectral-domain optical coherence tomography (SD-OCT). Time-lapse sagittal SD-OCT sections through Schlemm's canal (SC) were acquired while changing IOP stepwise between 10 and 45 mm Hg. After topical application of 1% pilocarpine, the series of IOP steps and imaging were repeated. Effects of pilocarpine on IOP and outflow facility in living mice were verified by rebound tonometry and flow measurements at three different IOPs, respectively. In vivo OCT images were compared with eyes analyzed by standard histology.
resultsIn living mice imaged by SD-OCT, the lumen of SC progressively collapsed with increasing IOP, reaching near complete closure at 20 mm Hg. Schlemm's canal collapse was reversible, with the lumen opening within minutes after returning IOP from 45 to 10 mm Hg. Pilocarpine-induced ciliary muscle contraction changed SC lumen area by 131.6% ± 21.0% compared with untreated controls at 10 mm Hg, opened the trabecular meshwork, and prevented complete collapse of the SC lumen at higher pressures. Similar results were observed by standard histology. Pilocarpine increased outflow facility 4-fold (P = 0.02) and lowered IOP (16.46 ± 2.23 vs. 11.08 ± 2.28 mm Hg, P = 0.03).
conclusionsSpectral-domain OCT was effective at visualizing changes in SC lumen in living mice. Results with pilocarpine are consistent with the concept that a primary role for the ciliary muscle is to prevent collapse of SC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.