ArticlePloS one2014
Extremely low-frequency electromagnetic fields affect transcript levels of neuronal differentiation-related genes in embryonic neural stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Mapping the Landscape of Magnetic Field Effects on Neural Regeneration and Repair: A Combined Systematic Review, Mathematical Model, and Meta-Analysis.Journal of tissue engineering and regenerative medicine · 2023Pooled it
- Trial
- The Frequency of a Magnetic Field Reduces the Viability and Proliferation of Numerous Tumor Cell Lines.Biomolecules · 2025Article
- The Frequency of a Magnetic Field Determines the Behavior of Tumor and Non-Tumor Nerve Cell Models.International journal of molecular sciences · 2025Article
- The Cellular Response Is Determined by a Combination of Different ELF-EMF Exposure Parameters: A Scope Review.International journal of molecular sciences · 2024Review
- Pulsating Extremely Low-Frequency Electromagnetic Fields Influence Differentiation of Mouse Neural Stem Cells towards Astrocyte-like Phenotypes: In Vitro Pilot Study.International journal of molecular sciences · 2024Article
- A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes.Biomedicines · 2022Article
- Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.Histochemistry and cell biology · 2022Article
- Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients.Brain sciences · 2020Article
- CT2A Cell Viability Modulated by Electromagnetic Fields at Extremely Low Frequency under No Thermal Effects.International journal of molecular sciences · 2019Article
- Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation.Journal of neural transmission (Vienna, Austria : 1996) · 2019Article
- Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation and Differentiation after Intracerebral Hemorrhage in Mice.Cell transplantation · 2019Article
- Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells.Iranian journal of basic medical sciences · 2019Article
- Static Magnetic Field Induced Neural Stem/Progenitor Cell Early Differentiation and Promotes Maturation.Stem cells international · 2019Article
- Regulation of Redox Homeostasis by Nonthermal Biocompatible Plasma Discharge in Stem Cell Differentiation.Oxidative medicine and cellular longevity · 2019Review
- ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis.Scientific reports · 2017Article
- Electromagnetic Fields for the Regulation of Neural Stem Cells.Stem cells international · 2017Review
- Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study.BMC complementary and alternative medicine · 2016Article
- Extremely Low Frequency Magnetic Field (ELF-MF) Exposure Sensitizes SH-SY5Y Cells to the Pro-Parkinson's Disease Toxin MPP(.).Molecular neurobiology · 2016Article
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Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have reported that extremely low-frequency electromagnetic fields (ELF-EMF) can affect the processes of brain development, but the underlying mechanism is largely unknown. The proliferation and differentiation of embryonic neural stem cells (eNSCs) is essential for brain development during the gestation period. To date, there is no report about the effects of ELF-EMF on eNSCs. In this paper, we studied the effects of ELF-EMF on the proliferation and differentiation of eNSCs. Primary cultured eNSCs were treated with 50 Hz ELF-EMF; various magnetic intensities and exposure times were applied. Our data showed that there was no significant change in cell proliferation, which was evaluated by cell viability (CCK-8 assay), DNA synthesis (Edu incorporation), average diameter of neurospheres, cell cycle distribution (flow cytometry) and transcript levels of cell cycle related genes (P53, P21 and GADD45 detected by real-time PCR). When eNSCs were induced to differentiation, real-time PCR results showed a down-regulation of Sox2 and up-regulation of Math1, Math3, Ngn1 and Tuj1 mRNA levels after 50 Hz ELF-EMF exposure (2 mT for 3 days), but the percentages of neurons (Tuj1 positive cells) and astrocytes (GFAP positive cells) were not altered when detected by immunofluorescence assay. Although cell proliferation and the percentages of neurons and astrocytes differentiated from eNSCs were not affected by 50 Hz ELF-EMF, the expression of genes regulating neuronal differentiation was altered. In conclusion, our results support that 50 Hz ELF-EMF induce molecular changes during eNSCs differentiation, which might be compensated by post-transcriptional mechanisms to support cellular homeostasis.
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