ArticleNature communications2014
Microfluidic probe for single-cell analysis in adherent tissue culture.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 96 citations in OpenAlex.
- Pixelated Microfluidics for Drug Screening on Tumour Spheroids and Ex Vivo Microdissected Tumour Explants.Cancers · 2023Article
- Spatial protein heterogeneity analysis in frozen tissues to evaluate tumor heterogeneity.PloS one · 2021Article
- Reagent integration and controlled release for multiplexed nucleic acid testing in disposable thermoplastic 2D microwell arrays.Biomicrofluidics · 2021Article
- A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells.Micromachines · 2020Article
- Step-defect guided delivery of DNA to a graphene nanopore.Nature nanotechnology · 2019Article
- Recent advances in microfluidic platforms for single-cell analysis in cancer biology, diagnosis and therapy.Trends in analytical chemistry : TRAC · 2019Article
- Microfluidic multipoles theory and applications.Nature communications · 2019Article
- Adhesion analysis of single circulating tumor cells on a base layer of endothelial cells using open microfluidics.Chemical science · 2018Article
- 3D Printed Microfluidic Probes.Scientific reports · 2018Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Continuous and High-Throughput Electromechanical Lysis of Bacterial Pathogens Using Ion Concentration Polarization.Analytical chemistry · 2018Article
- Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Platform for combined analysis of functional and biomolecular phenotypes of the same cell.Scientific reports · 2017Article
- Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Ultrasensitive detection of telomerase activity in a single cell using stem-loop primer-mediated exponential amplification (SPEA) with near zero nonspecific signal.Chemical science · 2016Article
- Selective local lysis and sampling of live cells for nucleic acid analysis using a microfluidic probe.Scientific reports · 2016Article
- Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer.Biomedical microdevices · 2016Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Spatial Chemical Stimulation Control in Microenvironment by Microfluidic Probe Integrated Device for Cell-Based Assay.PloS one · 2016Article
- Methods to study the tumor microenvironment under controlled oxygen conditions.Trends in biotechnology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Single-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.