ArticlePloS one2014
Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 166 citations in OpenAlex.
- Airborne Bacterial and Eukaryotic Communities in Temuco, Chile, a City Dominated by Residential Wood Combustion.Life (Basel, Switzerland) · 2026Article
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- Targeted molecular screening for protozoan parasites in wild platypuses (International journal for parasitology. Parasites and wildlife · 2025Article
- Beyond dikarya: 28S metabarcoding uncovers cryptic fungal lineages across a tidal estuary.Environmental microbiome · 2025Article
- A novel two-step metabarcoding approach improves soil microbiome biodiversity assessment.Scientific reports · 2025Article
- Accurate detection of tandem repeats exposes ubiquitous reuse of biological sequences.Nucleic acids research · 2025Article
- DNA Metabarcoding Focusing on Eukaryote Communities on Langhovde Glacier, East Antarctica.Environmental microbiology reports · 2025Article
- Molecular studies reveal host plasticity and wide geographical distribution ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Multi-marker comparative analysis of 18S, ITS1, and ITS2 primers for human gut mycobiome profiling.Frontiers in bioinformatics · 2025Article
- Algae-Mediated Green Synthesis of Dextran-Coated Titanium Nanoparticles and Their Cytotoxic Potential Against MCF7 Breast Cancer Cells.Reports of biochemistry & molecular biology · 2024Article
- PrimerEvalPy: a tool for in-silico evaluation of primers for targeting the microbiome.BMC bioinformatics · 2024Article
- Rice crop residue management by the microbial consortium for rapid decomposition of straw.3 Biotech · 2024Article
- Morphometric and Molecular Analysis of Five-SpineLife (Basel, Switzerland) · 2023Article
- First morphometric and molecular characterization of Fasciola spp. in Northwest Tunisia.Parasitology research · 2023Article
- Article
- Genome-Wide Analysis of MYB Transcription Factors and Screening ofInternational journal of molecular sciences · 2023Article
- First insights into the gut microbiomes and the diet of theEvolutionary applications · 2023Article
- The Overlooked Microbiome-Considering Archaea and Eukaryotes Using Multiplex Nanopore-16S-/18S-rDNA-Sequencing: A Technical Report Focusing on Nasopharyngeal Microbiomes.International journal of molecular sciences · 2023Article
- From Genomics to Metagenomics in the Era of Recent Sequencing Technologies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Thriving beneath olive trees: The influence of organic farming on microbial communities.Computational and structural biotechnology journal · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems. Here, we predicted 18S rRNA primers based on consecutive conserved sites and evaluated their coverage efficiency and scope of application to different eukaryotic groups. After evaluation, eight of them were considered as qualified 18S primers based on coverage rate. Next, we examined common conserved regions in prokaryotic 16S and eukaryotic 18S rRNA sequences to design 16S/18S universal primers. Three 16S/18S candidate primers, U515, U1390 and U1492, were then considered to be suitable for simultaneous amplification of the rRNA sequences in three domains. Eukaryotic 18S and prokaryotic 16S rRNA genes in a sponge were amplified simultaneously using universal primers U515 and U1390, and the subsequent sorting of pyrosequenced reads revealed some distinctive communities in different parts of the sample. The real difference in biodiversity between prokaryotic and eukaryotic symbionts could be discerned as the dissimilarity between OTUs was increased from 0.005 to 0.1. A network of the communities in external and internal parts of the sponge illustrated the co-variation of some unique microbes in certain parts of the sponge, suggesting that the universal primers are useful in simultaneous detection of prokaryotic and eukaryotic microbial communities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.