ArticleProceedings of the National Academy of Sciences of the United States of America2014
Gene silencing and gene expression in phytopathogenic fungi using a plant virus vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Nanoparticles and Nanocarriers for Managing Plant Viral Diseases.Plants (Basel, Switzerland) · 2025Review
- Transmission of apple stem grooving virus (Capillovirus mali) to apple from the soil-borne fungus Fusarium solani.BMC plant biology · 2025Article
- Tracking of Tobacco Mosaic Virus in Taxonomically Different Plant Fungi.Journal of fungi (Basel, Switzerland) · 2025Article
- Viral tropism in plants, reproductive tissues, and seeds.Archives of microbiology · 2025Review
- A novel tobamo-like mycovirus with filamentous particles replicates in plant cells.Journal of virology · 2025Article
- Meta-transcriptomic analysis reveals the geographical expansion of known sugarbeet-infecting viruses and the occurrence of a novel virus in sugarbeet in the United States.Frontiers in plant science · 2024Article
- The intriguing phenomenon of cross-kingdom infections of plant and insect viruses to fungi: Can other animal viruses also cross-infect fungi?PLoS pathogens · 2023Article
- Article
- Similar Characteristics of siRNAs of Plant Viruses Which Replicate in Plant and Fungal Hosts.Biology · 2022Article
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- Review
- Facilitative and synergistic interactions between fungal and plant viruses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi.Frontiers in microbiology · 2020Article
- Epigenetic mechanisms of drug resistance in fungi.Fungal genetics and biology : FG & B · 2019Review
- Symptomatic plant viroid infections in phytopathogenic fungi.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Tobacco mosaic virus infection triggers an RNAi-based response in Phytophthora infestans.Scientific reports · 2019Article
- Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes.PloS one · 2019Article
- Article
- TMV Particles: The Journey From Fundamental Studies to Bionanotechnology Applications.Advances in virus research · 2018Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a powerful approach for elucidating gene functions in a variety of organisms, including phytopathogenic fungi. In such fungi, RNAi has been induced by expressing hairpin RNAs delivered through plasmids, sequences integrated in fungal or plant genomes, or by RNAi generated in planta by a plant virus infection. All these approaches have some drawbacks ranging from instability of hairpin constructs in fungal cells to difficulties in preparing and handling transgenic plants to silence homologous sequences in fungi grown on these plants. Here we show that RNAi can be expressed in the phytopathogenic fungus Colletotrichum acutatum (strain C71) by virus-induced gene silencing (VIGS) without a plant intermediate, but by using the direct infection of a recombinant virus vector based on the plant virus, tobacco mosaic virus (TMV). We provide evidence that a wild-type isolate of TMV is able to enter C71 cells grown in liquid medium, replicate, and persist therein. With a similar approach, a recombinant TMV vector carrying a gene for the ectopic expression of the green fluorescent protein (GFP) induced the stable silencing of the GFP in the C. acutatum transformant line 10 expressing GFP derived from C71. The TMV-based vector also enabled C. acutatum to transiently express exogenous GFP up to six subcultures and for at least 2 mo after infection, without the need to develop transformation technology. With these characteristics, we anticipate this approach will find wider application as a tool in functional genomics of filamentous fungi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.