ArticleTissue engineering. Part A2014
Implanted cell-dense prevascularized tissues develop functional vasculature that supports reoxygenation after thrombosis.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Topological Tracks Patterned via 3D Printing Vascularize Murine Organ-Scale Constructs.Cell biomaterials · 2026Article
- bFGF-Loaded PDA Microparticles Enhance Vascularization of Engineered Skin with a Concomitant Increase in Leukocyte Recruitment.Bioengineering (Basel, Switzerland) · 2026Article
- 3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems.Science advances · 2025Article
- Multiscale Imaging to Monitor Functional SHED-Supported Engineered Vessels.Journal of dental research · 2024Article
- Article
- Development of fibroblast/endothelial cell-seeded collagen scaffolds for in vitro prevascularization.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Engineered tissue vascularization and engraftment depends on host model.Scientific reports · 2023Article
- Programming the Self-Organization of Endothelial Cells into Perfusable Microvasculature.Tissue engineering. Part A · 2023Article
- Biodegradable Inks in Indirect Three-Dimensional Bioprinting for Tissue Vascularization.Frontiers in bioengineering and biotechnology · 2022Review
- Engineering the multiscale complexity of vascular networks.Nature reviews. Materials · 2022Review
- Tissues with Patterned Vessels or Protein Release Induce Vascular Chemotaxis in anTissue engineering. Part A · 2021Article
- Blood Flow Within Bioengineered 3D Printed Vascular Constructs Using the Porcine Model.Frontiers in cardiovascular medicine · 2021Article
- Article
- Guided vascularization in the rat heart leads to transient vessel patterning.APL bioengineering · 2020Article
- Mature vessel networks in engineered tissue promote graft-host anastomosis and prevent graft thrombosis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Inspired by Nature: Hydrogels as Versatile Tools for Vascular Engineering.Advances in wound care · 2018Review
- Integrated approaches to spatiotemporally directing angiogenesis in host and engineered tissues.Acta biomaterialia · 2018Review
- Article
- Tissue Engineering the Vascular Tree.Tissue engineering. Part B, Reviews · 2017Review
- Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Achieving adequate vascularization within implanted engineered tissues is a significant obstacle to maintaining viability and functionality. In vitro prevascularization of engineered tissues has been explored as a potential solution to this challenge. The traditional paradigm of in vitro prevascularization is to implant an engineered tissue with a preformed vascular network that is perfused after anastomosis with the host circulation. We investigated the efficacy of this strategy by implanting cell-dense prevascularized tissues created via cell-mediated contraction and composed of collagen and a collagen-fibrin mixture into dorsal window chambers surgically prepared on immunocompromised mice. We found that host-implant anastomosis takes place in 2-6 days and that perfusion of vessels within the implants is subsequently restricted by thrombosis. However, by day 7, a functional vascular network composed of host and implant vessels developed. Prevascularization enhanced intra-implant pO2 significantly as early as 2 days postimplantation, reaching a maximum of 55 mmHg by day 8, which was significantly greater than the maximum within cellularized control tissues (18 mmHg). By day 14, collagen tissues supported ∼ 0.51 × 10(9) implanted and host-derived cells per mL. Our findings elucidate key features of in vitro prevascularization that can be used toward the design of larger and more functionally complex engineered tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.