ArticleBioMed research international2014
Molecular characterization of a recombinant manganese superoxide dismutase from Lactococcus lactis M4.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Two-step purification of elastin-like polypeptide-fusion superoxide dismutase via hydrophobicity and thermoresponsiveness.Frontiers in bioengineering and biotechnology · 2025Article
- CaMicrobiology spectrum · 2024Article
- Purification and Immobilization of Superoxide Dismutase Obtained fromBiomolecules · 2023Article
- Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium.Scientific reports · 2020Article
- Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromate.BMC microbiology · 2016Article
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Authors and funding
4 authors.
Funding
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Abstract
A superoxide dismutase (SOD) gene of Lactococcus lactis M4 was cloned and expressed in a prokaryotic system. Sequence analysis revealed an open reading frame of 621 bp which codes for 206 amino acid residues. Expression of sodA under T7 promoter exhibited a specific activity of 4967 U/mg when induced with 1 mM of isopropyl-β-D-thiogalactopyranoside. The recombinant SOD was purified to homogeneity by immobilised metal affinity chromatography and Superose 12 gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analyses of the recombinant SOD detected a molecular mass of approximately 27 kDa. However, the SOD was in dimer form as revealed by gel filtration chromatography. The purified recombinant enzyme had a pI of 4.5 and exhibited maximal activity at 25°C and pH 7.2. It was stable up to 45°C. The insensitivity of this lactococcal SOD to cyanide and hydrogen peroxide established that it was a MnSOD. Although it has 98% homology to SOD of L. lactis IL1403, this is the first elucidated structure of lactococcal SOD revealing active sites containing the catalytic manganese coordinated by four ligands (H-27, H-82, D-168, and H-172).
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