Evidence map›Paper›PMID 24591982›Full record

ArticleJournal of the Turkish German Gynecological Association2011

Combination of swim-up and density gradient separation methods effectively eliminate DNA damaged sperm.

Surveen Ghumman, Satish Kumar Adiga, Dinesh Upadhya, Guruprasad Kalthur, Varshini Jayaraman, Satish Bola Rao, Pratap Kumar

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the Turkish German Gynecological Association, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Surveen GhummanClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Satish Kumar AdigaClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Dinesh UpadhyaClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Guruprasad KalthurClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Varshini JayaramanClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Satish Bola RaoDepartment of Radiobiology and Toxicology, Manipal Life Science Centre, Manipal, India.
Pratap KumarClinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.
Manipal Academy of Higher Education · INKasturba Medical College, Manipal · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. MATERIAL AND

methodsSperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated.

resultsThe amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population.

conclusionThe combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.

Indexed as

comet assaydensity gradient separationDNA damageSperm washswim-up

Identifiers

PMID24591982
PMCPMC3939271
OpenAlexW1983550878

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.