ArticleThe Journal of physiology2014
Subcellular distribution of glycogen and decreased tetanic Ca2+ in fatigued single intact mouse muscle fibres.
Article in The Journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
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- Isoenergetic Pre-Exercise Meals Varying in Carbohydrate Similarly Affect Resistance Training Volume Performance Compared to Placebo: A Crossover Trial.European journal of sport science · 2025Trial
- Local depletion of glycogen with supramaximal exercise in human skeletal muscle fibres.The Journal of physiology · 2017Trial
- Low-dose lithium supplementation promotes musculoskeletal and metabolic health in ovariectomized female mice.The Journal of physiology · 2026Article
- Carbohydrate ingestion during prolonged exercise blunts the reduction in power output at the moderate-to-heavy intensity transition.European journal of applied physiology · 2025Article
- Evaluation of the association of carbohydrate quality on enjoyment of physical activity and burnout in athletes.BMC sports science, medicine & rehabilitation · 2025Article
- Durability of the moderate-to-heavy-intensity transition is related to the effects of prolonged exercise on severe-intensity performance.European journal of applied physiology · 2024Article
- The addition of blood flow restriction during resistance exercise does not increase prolonged low-frequency force depression.Experimental physiology · 2024Article
- Numerous Trigger-like Interactions of Kinases/Protein Phosphatases in Human Skeletal Muscles Can Underlie Transient Processes in Activation of Signaling Pathways during Exercise.International journal of molecular sciences · 2023Review
- New Horizons in Carbohydrate Research and Application for Endurance Athletes.Sports medicine (Auckland, N.Z.) · 2022Review
- Fibre type- and localisation-specific muscle glycogen utilisation during repeated high-intensity intermittent exercise.The Journal of physiology · 2022Article
- The spatial distribution of glycogen and glycogen consumption in muscle cells.The Journal of general physiology · 2022Article
- Specific ATPases drive compartmentalized glycogen utilization in rat skeletal muscle.The Journal of general physiology · 2022Article
- Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Muscle Glycogen Metabolism and High-Intensity Exercise Performance: A Narrative Review.Sports medicine (Auckland, N.Z.) · 2021Review
- Re-Evaluating the Oxidative Phenotype: Can Endurance Exercise Save the Western World?Antioxidants (Basel, Switzerland) · 2021Review
- Fast skeletal muscle troponin activator CK-2066260 mitigates skeletal muscle weakness independently of the underlying cause.Journal of cachexia, sarcopenia and muscle · 2020Article
- Comment on: "Changes in Skeletal Muscle Glycogen Content in Professional Soccer Players before and after a Match by a NonInvasive MuscleSoundNutrients · 2020Article
- Article
- Effects of reduced muscle glycogen on excitation-contraction coupling in rat fast-twitch muscle: a glycogen removal study.Journal of muscle research and cell motility · 2019Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In skeletal muscle fibres, glycogen has been shown to be stored at different subcellular locations: (i) between the myofibrils (intermyofibrillar); (ii) within the myofibrils (intramyofibrillar); and (iii) subsarcolemmal. Of these, intramyofibrillar glycogen has been implied as a critical regulator of sarcoplasmic reticulum Ca(2+) release. The aim of the present study was to test directly how the decrease in cytoplasmic free Ca(2+) ([Ca(2+)]i) during repeated tetanic contractions relates to the subcellular glycogen distribution. Single fibres of mouse flexor digitorum brevis muscles were fatigued with 70 Hz, 350 ms tetani given at 2 s (high-intensity fatigue, HIF) or 10 s (low-intensity fatigue, LIF) intervals, while force and [Ca(2+)]i were measured. Stimulation continued until force decreased to 30% of its initial value. Fibres were then prepared for analyses of subcellular glycogen distribution by transmission electron microscopy. At fatigue, tetanic [Ca(2+)]i was reduced to 70 ± 4% and 54 ± 4% of the initial in HIF (P < 0.01, n = 9) and LIF (P < 0.01, n = 5) fibres, respectively. At fatigue, the mean inter- and intramyofibrillar glycogen content was 60-75% lower than in rested control fibres (P < 0.05), whereas subsarcolemmal glycogen was similar to control. Individual fibres showed a good correlation between the fatigue-induced decrease in tetanic [Ca(2+)]i and the reduction in intermyofibrillar (P = 0.051) and intramyofibrillar (P = 0.0008) glycogen. In conclusion, the fatigue-induced decrease in tetanic [Ca(2+)]i, and hence force, is accompanied by major reductions in inter- and intramyofibrillar glycogen. The stronger correlation between decreased tetanic [Ca(2+)]i and reduced intramyofibrillar glycogen implies that sarcoplasmic reticulum Ca(2+) release critically depends on energy supply from the intramyofibrillar glycogen pool.
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