ArticleThe Journal of physiology2014
Novel approaches to determine contractile function of the isolated adult zebrafish ventricular cardiac myocyte.
Article in The Journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Myosin-binding protein C slows cardiac myofibril relaxation kinetics.The Journal of physiology · 2025Article
- Impact of stretch on sarcomere length variability in isolated fully relaxed rat cardiac myocytes.Pflugers Archiv : European journal of physiology · 2023Article
- Impact of stretch on sarcomere length variability in isolated fully relaxed rat cardiac myocytes.Research square · 2023Article
- Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin-Biotin Affinity.ACS omega · 2021Article
- Modeling Inherited Cardiomyopathies in Adult Zebrafish for Precision Medicine.Frontiers in physiology · 2020Review
- Haploinsufficiency of mechanistic target of rapamycin amelioratesDisease models & mechanisms · 2019Article
- Phenotyping an adult zebrafish lamp2 cardiomyopathy model identifies mTOR inhibition as a candidate therapy.Journal of molecular and cellular cardiology · 2019Article
- Phenotyping cardiomyopathy in adult zebrafish.Progress in biophysics and molecular biology · 2018Review
- A Langendorff-like system to quantify cardiac pump function in adult zebrafish.Disease models & mechanisms · 2018Article
- Restrictive Cardiomyopathy Troponin I R145W Mutation Does Not Perturb Myofilament Length-dependent Activation in Human Cardiac Sarcomeres.The Journal of biological chemistry · 2016Article
- Zebrafish heart as a model to study the integrative autonomic control of pacemaker function.American journal of physiology. Heart and circulatory physiology · 2016Article
- Instrumentation to study myofibril mechanics from static to artificial simulations of cardiac cycle.MethodsX · 2016Article
- Construction and use of a zebrafish heart voltage and calcium optical mapping system, with integrated electrocardiogram and programmable electrical stimulation.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Hook, line and sinker: adult zebrafish offer a valid model to study mammalian cardiac contractile mechanics.The Journal of physiology · 2014Article
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5 authors.
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Abstract
The zebrafish (Danio rerio) has been used extensively in cardiovascular biology, but mainly in the study of heart development. The relative ease of its genetic manipulation may indicate the suitability of this species as a cost-effective model system for the study of cardiac contractile biology. However, whether the zebrafish heart is an appropriate model system for investigations pertaining to mammalian cardiac contractile structure-function relationships remains to be resolved. Myocytes were isolated from adult zebrafish hearts by enzymatic digestion, attached to carbon rods, and twitch force and intracellular Ca(2+) were measured. We observed the modulation of twitch force, but not of intracellular Ca(2+), by both extracellular [Ca(2+)] and sarcomere length. In permeabilized cells/myofibrils, we found robust myofilament length-dependent activation. Moreover, modulation of myofilament activation-relaxation and force redevelopment kinetics by varied Ca(2+) activation levels resembled that found previously in mammalian myofilaments. We conclude that the zebrafish is a valid model system for the study of cardiac contractile structure-function relationships.
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