ArticleThe Journal of cell biology1988
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed, 207 citations in OpenAlex.
- Methods to separate nuclear soluble fractions reflecting localizations in living cells.iScience · 2021Article
- Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy.Viruses · 2021Review
- Nuclear import of adenovirus DNA involves direct interaction of hexon with an N-terminal domain of the nucleoporin Nup214.Journal of virology · 2015Article
- Article
- Functional lipids and lipoplexes for improved gene delivery.Biochimie · 2012Review
- Using single-particle tracking to study nuclear trafficking of viral genes.Biophysical journal · 2004Article
- Antigen transport and cytoskeletal characteristics of a distinct enterocyte population in inflammatory bowel diseases.The American journal of pathology · 2004Article
- The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.The Journal of cell biology · 2002Article
- ERK2 enters the nucleus by a carrier-independent mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay.Molecular biology of the cell · 2000Article
- Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms.The Journal of cell biology · 2000Article
- Nuclear export in plants. Use of geminivirus movement proteins for a cell-based export assay.The Plant cell · 1999Article
- The nucleoporin nup153 plays a critical role in multiple types of nuclear export.Molecular biology of the cell · 1999Article
- The nuclear pore complex.Plant molecular biology · 1998Review
- Transport of macromolecules between the nucleus and the cytoplasm.RNA (New York, N.Y.) · 1998Review
- Dynamic equilibrium between calcineurin and kinase activities regulates the phosphorylation state and localization of the nuclear factor of activated T-cells.The Biochemical journal · 1997Article
- The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.The Journal of cell biology · 1997Article
- Targeting and function in mRNA export of nuclear pore complex protein Nup153.The Journal of cell biology · 1996Article
- A GTPase distinct from Ran is involved in nuclear protein import.The Journal of cell biology · 1996Article
- Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex.Molecular and cellular biology · 1996Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react with similar components in Xenopus oocytes, as determined by immunofluorescence microscopy and immunoblotting. We have microinjected the antibody into oocytes to study the possible role of these polypeptides in nucleocytoplasmic transport. The antibody inhibits import of a large nuclear protein, nucleoplasmin, in a time- and concentration-dependent manner. It also inhibits export of 5S ribosomal RNA and mature tRNA, but has no effect on transcription or intranuclear tRNA processing. The antibody does not affect the rate of diffusion into the nucleus of two small proteins, myoglobin and ovalbumin, indicating that antibody binding does not result in occlusion of the channel for diffusion. This suggests that inhibition of protein and RNA transport occurs by binding of the antibody at or near components of the pore that participate in mediated transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.