ArticlePlant physiology2014
Metabolic flux analysis of plastidic isoprenoid biosynthesis in poplar leaves emitting and nonemitting isoprene.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 136 citations in OpenAlex.
- Metabolite control of enzyme activity links stress to biosynthetic regulation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Volatile-Mediated Plant Defense Networks: Field Evidence for Isoprene as a Short-Distance Immune Signal.Plant, cell & environment · 2025Article
- Abscisic acid modulation of drought tolerance and essential oil biosynthesis in Lavandula Coronopifolia Poir.Scientific reports · 2025Article
- Photosynthetic Temperature Tolerance Threshold Determines How Isoprene Emission is Affected by Elevated COPlant-environment interactions (Hoboken, N.J.) · 2025Article
- Deoxyxylulose 5-Phosphate Synthase Does Not Play a Major Role in Regulating the Methylerythritol 4-Phosphate Pathway in Poplar.International journal of molecular sciences · 2024Article
- A Novel Isoprene Synthase from the Monocot TreeInternational journal of molecular sciences · 2023Article
- HDR, the last enzyme in the MEP pathway, differently regulates isoprenoid biosynthesis in two woody plants.Plant physiology · 2023Article
- MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply.Plant communications · 2023Article
- Isoprene-Emitting Tobacco Plants Are Less Affected by Moderate Water Deficit under Future Climate Change Scenario and Show Adjustments of Stress-Related Proteins in Actual Climate.Plants (Basel, Switzerland) · 2023Article
- Combination of long-termPlant methods · 2022Article
- Isoprene Emission Influences the Proteomic Profile of Arabidopsis Plants under Well-Watered and Drought-Stress Conditions.International journal of molecular sciences · 2022Article
- Exposure to different light intensities affects emission of volatiles and accumulations of both pigments and phenolics in Azolla filiculoides.Physiologia plantarum · 2022Article
- Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways.Frontiers in plant science · 2022Article
- Protein expression plasticity contributes to heat and drought tolerance of date palm.Oecologia · 2021Article
- Heatwave frequency and seedling death alter stress-specific emissions of volatile organic compounds in Aleppo pine.Oecologia · 2021Article
- Physiological response and secondary metabolites of three lavender genotypes under water deficit.Scientific reports · 2021Article
- Review
- Isoprene: An Antioxidant Itself or a Molecule with Multiple Regulatory Functions in Plants?Antioxidants (Basel, Switzerland) · 2021Review
- Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.Plant methods · 2021Article
- Proteostatic Regulation of MEP and Shikimate Pathways by Redox-Activated Photosynthesis Signaling in Plants Exposed to Small Fungal Volatiles.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastidic 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway is one of the most important pathways in plants and produces a large variety of essential isoprenoids. Its regulation, however, is still not well understood. Using the stable isotope 13C-labeling technique, we analyzed the carbon fluxes through the MEP pathway and into the major plastidic isoprenoid products in isoprene-emitting and transgenic isoprene-nonemitting (NE) gray poplar (Populus×canescens). We assessed the dependence on temperature, light intensity, and atmospheric [CO2]. Isoprene biosynthesis was by far (99%) the main carbon sink of MEP pathway intermediates in mature gray poplar leaves, and its production required severalfold higher carbon fluxes compared with NE leaves with almost zero isoprene emission. To compensate for the much lower demand for carbon, NE leaves drastically reduced the overall carbon flux within the MEP pathway. Feedback inhibition of 1-deoxy-D-xylulose-5-phosphate synthase activity by accumulated plastidic dimethylallyl diphosphate almost completely explained this reduction in carbon flux. Our data demonstrate that short-term biochemical feedback regulation of 1-deoxy-d-xylulose-5-phosphate synthase activity by plastidic dimethylallyl diphosphate is an important regulatory mechanism of the MEP pathway. Despite being relieved from the large carbon demand of isoprene biosynthesis, NE plants redirected only approximately 0.5% of this saved carbon toward essential nonvolatile isoprenoids, i.e. β-carotene and lutein, most probably to compensate for the absence of isoprene and its antioxidant properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.