ArticleProtoplasma2014
A new high-frequency Agrobacterium-mediated transformation technique for Sesamum indicum L. using de-embryonated cotyledon as explant.
Article in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Cloning and Expression Studies of Osmotin-Like Protein Gene from Solanum nigrum in Escherichia coli.Molecular biotechnology · 2026Article
- Genetic engineering and genome editing technologies as catalyst for Africa's food security: the case of plant biotechnology in Nigeria.Frontiers in genome editing · 2024Review
- Stable In-Planta Transformation System For Egyptian Sesame (GM crops & food · 2023Article
- Lignans of Sesame (Molecules (Basel, Switzerland) · 2021Review
- Biotrophy-necrotrophy switch in pathogen evoke differential response in resistant and susceptible sesame involving multiple signaling pathways at different phases.Scientific reports · 2017Article
- Induction of shoot regeneration in cotyledon explants of the oilseed cropJournal, genetic engineering & biotechnology · 2017Article
- The Emerging Oilseed CropFrontiers in plant science · 2017Review
- Overexpression of a New Osmotin-Like Protein Gene (Frontiers in plant science · 2017Article
- Cloning, characterization, and bacterial over-expression of an osmotin-like protein gene from Solanum nigrum L. with antifungal activity against three necrotrophic fungi.Molecular biotechnology · 2015Article
- Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops.Plant molecular biology · 2014Article
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Authors and funding
3 authors.
Funding
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Abstract
In spite of the economic importance of sesame (Sesamum indicum L.) and the recent availability of its genome sequence, a high-frequency transformation protocol is still not available. The only two existing Agrobacterium-mediated transformation protocols that are available have poor transformation efficiencies of less than 2%. In the present study, we report a high-frequency, simple, and reproducible transformation protocol for sesame. Transformation was done using de-embryonated cotyledons via somatic embryogenic stages. All the critical parameters of transformation, like incubation period of explants in pre-regeneration medium prior to infection by Agrobacterium tumefaciens, cocultivation period, concentrations of acetosyringone in cocultivation medium, kanamycin concentration, and concentration of plant hormones, including 6-benzylaminopurine, have been optimized. This protocol is superior to the two existing protocols in its high regeneration and transformation efficiencies. The transformed sesame lines have been tested by PCR, RT-PCR for neomycin phosphotransferase II gene expression, and β-glucuronidase (GUS) assay. The regeneration frequency and transformation efficiency are 57.33 and 42.66%, respectively. T0 and T1 generation transgenic plants were analyzed, and several T1 plants homozygous for the transgenes were obtained.
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