ArticleThe Journal of cell biology2014
Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 83 citations in OpenAlex.
- Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1.Nature communications · 2026Article
- McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylation.EMBO reports · 2026Article
- Advances in high-resolution imaging of centrioles and cilia.Journal of cell science · 2025Review
- The A-C linker controls centriole structural integrity and duplication.Nature communications · 2025Article
- Visualizing Cartwheel Disassembly Process During Mitosis in Fixed and Live Cells by Fluorescence Microscope.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM.EMBO reports · 2024Article
- Single-molecule tracking to determine the abundances and stoichiometries of freely-diffusing protein complexes in living cells: Past applications and future prospects.The Journal of chemical physics · 2023Review
- The E3 ligase Poe promotes Pericentrin degradation.Molecular biology of the cell · 2023Article
- GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells.Life science alliance · 2023Article
- Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.The Journal of cell biology · 2022Article
- Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel.Structure (London, England : 1993) · 2022Article
- Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture.Nature communications · 2021Article
- Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells.Molecular biology of the cell · 2021Article
- A first-takes-all model of centriole copy number control based on cartwheel elongation.PLoS computational biology · 2021Article
- Molecular Genetics of Microcephaly Primary Hereditary: An Overview.Brain sciences · 2021Review
- PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.The Journal of cell biology · 2021Article
- Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization.The Journal of biological chemistry · 2020Article
- A self-assembled cylindrical platform for Plk4-induced centriole biogenesis.Open biology · 2020Review
- Dissecting the Genetic and Etiological Causes of Primary Microcephaly.Frontiers in neurology · 2020Review
- Phase separation of Polo-like kinase 4 by autoactivation and clustering drives centriole biogenesis.Nature communications · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 are each required for procentriole formation in human cells. Moreover, the coiled coil is necessary and sufficient to mediate HsSAS-6 centrosomal targeting. High-resolution imaging reveals that GFP-tagged HsSAS-6 variants localize in a torus around the base of the parental centriole before S phase, perhaps indicative of an initial loading platform. Moreover, fluorescence recovery after photobleaching analysis demonstrates that HsSAS-6 is immobilized progressively at centrosomes during cell cycle progression. Using fluorescence correlation spectroscopy and three-dimensional stochastic optical reconstruction microscopy, we uncover that HsSAS-6 is present in the cytoplasm primarily as a homodimer and that its oligomerization into a ninefold symmetrical ring occurs at centrioles. Together, our findings lead us to propose a mechanism whereby HsSAS-6 homodimers are targeted to centrosomes where the local environment and high concentration of HsSAS-6 promote oligomerization, thus initiating procentriole formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.