ArticleClinical immunology (Orlando, Fla.)
Identifying functional anti-Staphylococcus aureus antibodies by sequencing antibody repertoires of patient plasmablasts.
Article in Clinical immunology (Orlando, Fla.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Medical software for precision diagnostics of infection with immunoprofiling and artificial intelligence.Journal of translational medicine · 2026Review
- Single-cell Sequencing of Circulating Human Plasmablasts during Staphylococcus aureus Bacteremia.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Impaired Antibody-Dependent Cellular Cytotoxicity in a Spanish Cohort of Patients With COVID-19 Admitted to the ICU.Frontiers in immunology · 2021Observational
- Prospective Artificial Intelligence to Dissect the Dengue Immune Response and Discover Therapeutics.Frontiers in immunology · 2021Review
- Pattern of circulating SARS-CoV-2-specific antibody-secreting and memory B-cell generation in patients with acute COVID-19.Clinical & translational immunology · 2021Article
- A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease.The Journal of infectious diseases · 2020Article
- B cells in rheumatoid arthritis synovial tissues encode focused antibody repertoires that include antibodies that stimulate macrophage TNF-α production.Clinical immunology (Orlando, Fla.) · 2020Article
- Longitudinal analysis of acute and convalescent B cell responses in a human primary dengue serotype 2 infection model.EBioMedicine · 2019Article
- Editorial: Reverse Vaccinology.Frontiers in immunology · 2019Article
- Affinity Maturation Drives Epitope Spreading and Generation of Proinflammatory Anti-Citrullinated Protein Antibodies in Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2018Article
- Circulating plasmablasts are elevated and produce pathogenic anti-endothelial cell autoantibodies in idiopathic pulmonary arterial hypertension.European journal of immunology · 2018Article
- Many Routes to an Antibody Heavy-Chain CDR3: Necessary, Yet Insufficient, for Specific Binding.Frontiers in immunology · 2018Article
- Robust B Cell Responses Predict Rapid Resolution of Lyme Disease.Frontiers in immunology · 2018Article
- Bacterial Vaccine Antigen Discovery in the Reverse Vaccinology 2.0 Era: Progress and Challenges.Frontiers in immunology · 2018Review
- VP4- and VP7-specific antibodies mediate heterotypic immunity to rotavirus in humans.Science translational medicine · 2017Article
- Next-Generation Techniques for Discovering Human Monoclonal Antibodies.Molecular biology · 2017Article
- Elevated IgA Plasmablast Levels in Subjects at Risk of Developing Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- High-quality full-length immunoglobulin profiling with unique molecular barcoding.Nature protocols · 2016Article
- Statistical inference of a convergent antibody repertoire response to influenza vaccine.Genome medicine · 2016Article
- The Diversity and Molecular Evolution of B-Cell Receptors during Infection.Molecular biology and evolution · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Infection by Staphylococcus aureus is on the rise, and there is a need for a better understanding of host immune responses that combat S. aureus. Here we use DNA barcoding to enable deep sequencing of the paired heavy- and light-chain immunoglobulin genes expressed by individual plasmablasts derived from S. aureus-infected humans. Bioinformatic analysis of the antibody repertoires revealed clonal families of heavy-chain sequences and enabled rational selection of antibodies for recombinant expression. Of the ten recombinant antibodies produced, seven bound to S. aureus, of which four promoted opsonophagocytosis of S. aureus. Five of the antibodies bound to known S. aureus cell-surface antigens, including fibronectin-binding protein A. Fibronectin-binding protein A-specific antibodies were isolated from two independent S. aureus-infected patients and mediated neutrophil killing of S. aureus in in vitro assays. Thus, our DNA barcoding approach enabled efficient identification of antibodies involved in protective host antibody responses against S. aureus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.