ArticleMethods and applications in fluorescence2013
Extending single molecule fluorescence observation time by amplitude-modulated excitation.
Article in Methods and applications in fluorescence, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Native diffusion of fluorogenic turn-on dyes accurately report interfacial chemical reaction locations.Analytical and bioanalytical chemistry · 2023Article
- Article
- Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.ACS nano · 2015Article
- Molecular approaches to chromatography using single molecule spectroscopy.Analytical chemistry · 2015Review
- Charge-dependent transport switching of single molecular ions in a weak polyelectrolyte multilayer.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- High ionic strength narrows the population of sites participating in protein ion-exchange adsorption: a single-molecule study.Journal of chromatography. A · 2014Article
- Troika of single particle tracking programing: SNR enhancement, particle identification, and mapping.Physical chemistry chemical physics : PCCP · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent data conversion time of the detector. The observation time and precision in super-localization of four commonly used fluorophores were compared under modulated and traditional continuous excitation, including direct total internal reflectance excitation of Alexa 555 and Cy3, non-radiative Förster resonance energy transfer (FRET) excited Cy5, and direct epi-fluorescence wide field excitation of Rhodamine 6G. The proposed amplitude-modulated excitation does not perturb the chemical makeup of the system or sacrifice signal and is compatible with multiple types of fluorophores. Amplitude-modulated excitation has practical applications for any fluorescent study utilizing an instrumental setup with time-delayed detectors.
Identifiers
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Registered trials
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