ArticleWorld journal of gastroenterology2014
Diet high in fructose leads to an overexpression of lipocalin-2 in rat fatty liver.
Article in World journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 87 citations in OpenAlex.
- The Role of High-Fructose Diet in Liver Function of Rodent Models: A Systematic Review of Molecular Analysis.Iranian biomedical journal · 2023Pooled it
- Investigating the therapeutic effects of caffeine-naringenin combination on the repair of intestinal permeability and composition of gut microbiota in obese rats under long-term high-fat diet.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Modulating the Gut-Liver Axis: Anti-Inflammatory Mechanisms of Probiotics and Prebiotics in MASLD.Probiotics and antimicrobial proteins · 2026Review
- Excessive intake of fructose and alcohol aggravates high-fat diet-induced steatohepatitis and changes hepatic iron homeostasis.Histochemistry and cell biology · 2025Article
- A novel mouse model for studying complications related to type 2 diabetes using a medium-fat diet, fructose, and streptozotocin.Scientific reports · 2025Article
- Induction of Fructose Mediated De Novo Lipogenesis Coexists with the Upregulation of Mitochondrial Oxidative Function in Mice Livers.The Journal of nutrition · 2025Article
- Temporary dietary fiber depletion prompts rapid and lasting gut microbiota restructuring in mice.Microbiology spectrum · 2025Article
- LCN2 depletion aggravates sepsis-induced liver injury by regulating PTGS2-dependent ferroptosis.International journal of medical sciences · 2024Article
- A Multidrug Donor Preconditioning Improves Steatotic Rat Liver Allograft Function and Recipient Survival After Transplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2024Article
- Article
- NLRP3 activation in neutrophils induces lethal autoinflammation, liver inflammation, and fibrosis.EMBO reports · 2022Article
- Relationship between Leptin and Insulin Resistance among Community-Dwelling Middle-Aged and Elderly Populations in Taiwan.Journal of clinical medicine · 2022Article
- Naringin attenuates fructose-induced NAFLD progression in rats through reducing endogenous triglyceride synthesis and activating the Nrf2/HO-1 pathway.Frontiers in pharmacology · 2022Article
- Excessive intake of sugar: An accomplice of inflammation.Frontiers in immunology · 2022Review
- MH-76, a Novel Non-Quinazoline αPharmaceuticals (Basel, Switzerland) · 2021Article
- Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia.Nature communications · 2021Article
- A Scoping Review on Lipocalin-2 and Its Role in Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma.International journal of molecular sciences · 2021Article
- Glucose transporters in adipose tissue, liver, and skeletal muscle in metabolic health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Automated computerized image analysis for the user-independent evaluation of disease severity in preclinical models of NAFLD/NASH.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Sex-specific metabolic functions of adipose Lipocalin-2.Molecular metabolism · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo explore lipocalin-2 (LCN-2) expression and its possible role and mechanism(s) of production in rat models of diet-inducible fatty liver.
methodsFatty liver was triggered in male Sprague-Dawley rats fed either with liquid Lieber-DeCarli (LDC) or LDC + 70% cal fructose (L-HFr) diet for 4 or 8 wk. Chow-nourished animals served as controls. Hepatic expression of LCN-2 and other metabolic and inflammatory mediators was assessed by quantitative reverse transcription polymerase chain reaction and Western blotting. Serum LCN-2, fasting leptin, and lipid profile were evaluated via Enzyme-Linked Immunosorbent Assay, Radioimmunoassay, and colorimetric assays, respectively. The localization of LCN-2 in the liver was detected by using immunofluorescence staining. Furthermore, HE stain was used to evaluate hepatic fat degeneration and inflammation.
resultsBoth LDC-fed and L-HFr-fed rat histologically featured fatty liver. In the liver, mRNA transcriptions of Mcp-1, a2-m, Il-8 and Glut5 were increased in the L-HFr group at both time points (P < 0.001), while the transcription of Tlr4, Inos, and Tnf-α was significantly up-regulated at week 4. Interestingly, hepatic Lcn-2 expression was 90-fold at week 4 and 507-fold at week 8 higher in L-HFr-subjected rats vs control (P < 0.001). In contrast to HDL-cholesterol, systemic levels of LCN-2, fasting leptin and triglycerides were elevated in the L-HFr regimen (P < 0.001). Moreover, protein expression of hepatic LCN-2, CD14, phospho-MAPK, caspase-9, cytochrome c and 4-hydroxynonenal was increased in the L-HFr group. Conversely, the hepatic expression of PGC-1α (a mitochondrial-biogenic protein) was reduced in the L-HFr category at week 8. The localization of LCN-2 in the liver was predominantly restricted to MPO⁺ granulocytes.
conclusionFructose diet up-regulates hepatic LCN-2 expression, which correlates with the increased indicators of oxidative stress and mitochondrial dysfunction. The LCN-2 may be involved in liver protection.
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