ArticlePLoS neglected tropical diseases2014
Comparative analysis of salivary gland transcriptomes of Phlebotomus orientalis sand flies from endemic and non-endemic foci of visceral leishmaniasis.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- First record of Phlebotomus (Larroussius) orientalis (Parrot, 1936) (Diptera: Psychodidae) in Israel: phylogeographic placement and implications for leishmaniasis surveillance.Parasites & vectors · 2026Article
- PpSP32-like protein as a marker of human exposure to Phlebotomus argentipes in Leishmania donovani foci in Bangladesh.International journal for parasitology · 2021Article
- Arboviruses: How Saliva Impacts the Journey from Vector to Host.International journal of molecular sciences · 2021Review
- Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi-proven vector of zoonotic cutaneous leishmaniasis in Iran.Veterinary medicine and science · 2021Article
- RNA-sequencing of the Nyssomyia neivai sialome: a sand fly-vector from a Brazilian endemic area for tegumentary leishmaniasis and pemphigus foliaceus.Scientific reports · 2020Article
- Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study.Parasites & vectors · 2020Article
- Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.Scientific reports · 2020Article
- Immunity to vector saliva is compromised by short sand fly seasons in endemic regions with temperate climates.Scientific reports · 2020Article
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- Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.PLoS neglected tropical diseases · 2019Article
- Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa.PLoS neglected tropical diseases · 2018Article
- Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9.PLoS neglected tropical diseases · 2018Article
- A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes.Insect biochemistry and molecular biology · 2018Article
- Comparative Evolution of Sand Fly Salivary Protein Families and Implications for Biomarkers of Vector Exposure and Salivary Vaccine Candidates.Frontiers in cellular and infection microbiology · 2018Article
- Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.PLoS neglected tropical diseases · 2017Review
- Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica.PLoS neglected tropical diseases · 2016Article
- Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites.PLoS neglected tropical diseases · 2016Article
- Hyaluronidase Activity in Saliva of European Culicoides (Diptera: Ceratopogonidae).Journal of medical entomology · 2016Article
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Corrections and comments
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Authors and funding
15 authors at 6 institutions in 4 countries.
Funding
Abstract
backgroundIn East Africa, Phlebotomus orientalis serves as the main vector of Leishmania donovani, the causative agent of visceral leishmaniasis (VL). Phlebotomus orientalis is present at two distant localities in Ethiopia; Addis Zemen where VL is endemic and Melka Werer where transmission of VL does not occur. To find out whether the difference in epidemiology of VL is due to distant compositions of P. orientalis saliva we established colonies from Addis Zemen and Melka Werer, analyzed and compared the transcriptomes, proteomes and enzymatic activity of the salivary glands. METHODOLOGY/PRINCIPAL
findingsTwo cDNA libraries were constructed from the female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Clones of each P. orientalis library were randomly selected, sequenced and analyzed. In P. orientalis transcriptomes, we identified members of 13 main protein families. Phylogenetic analysis and multiple sequence alignments were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. To further compare both colonies, we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase.
conclusionsThis is the first report of the salivary components of P. orientalis, an important vector sand fly. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. Based on the phylogenetic analysis, we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We also demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen (endemic area) and Melka Werer (non-endemic area) P. orientalis colonies. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition.
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