ArticlePLoS neglected tropical diseases2014
Parasite-derived microRNAs in host serum as novel biomarkers of helminth infection.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
82 citing papers in PubMed, 2 syntheses or guidelines pooled it, 155 citations in OpenAlex.
- MicroRNA Responses Associated with Vector-Borne Diseases in Humans and Arthropod Vectors: A Systematic Review.International journal of molecular sciences · 2026Pooled it
- MicroRNAs during Echinococcus spp. infection: a comprehensive systematic review and implications for diagnosis and therapy.Parasites & vectors · 2026Pooled it
- Host-parasite cross-species regulation by Schistosoma japonicum EV-miRNA sja-miR-2a-3p limits hepatic fibrosis via BCL2 suppression in hepatic stellate cells.Veterinary research · 2026Article
- Schistosome-derived microRNAs: promise, pitfalls and priorities for molecular diagnosis.Infectious diseases of poverty · 2026Article
- Detection of Echinococcus granulosus sensu lato microRNAs in cystic echinococcosis patients: An exploratory study using quantitative PCR and digital PCR.PLoS neglected tropical diseases · 2025Article
- Analysis of miR-10a and IFNG Expression Before and After Treatment for Chronic Schistosomiasis mansoni.Parasite immunology · 2025Article
- Comparative microRNAs profile of Schistosoma japonicum male worms derived from single-sex and bisexual infections: Implications of the multifunctional role of microRNA.Parasitology research · 2025Article
- Predictive multi-omic biomarkers for urban zoonotic spillover detection: an integrative review.Frontiers in public health · 2025Review
- Exploring microRNA-Mediated Immune Responses to Soil-Transmitted Helminth and Herpes Simplex Virus Type 2 Co-Infections.Diseases (Basel, Switzerland) · 2025Review
- Unraveling the microRNAs Involved in Fasciolosis: Master Regulators of the Host-Parasite Crosstalk.International journal of molecular sciences · 2024Review
- Assessment of gastrointestinal nematode infections in dairy cattle and the associated risk factors in Northern China.Journal of veterinary science · 2024Article
- Exploring the utility of circulating miRNAs as diagnostic biomarkers of fasciolosis.Scientific reports · 2024Article
- Comprehensive analysis of miRNA profiling in Schistosoma mekongi across life cycle stages.Scientific reports · 2024Article
- Non-coding RNA in the gut of the blood-feeding parasitic worm, Haemonchus contortus.Veterinary research · 2024Article
- Subtle transcriptomic response of Eurasian perch (International journal for parasitology. Parasites and wildlife · 2023Article
- microRNAs: Critical Players during Helminth Infections.Microorganisms · 2022Review
- Expression of microRNA-223 and microRNA-146b in serum and liver tissue of mice infected with Schistosoma mansoni.Parasitology research · 2022Article
- Extracellular vesicles-associated tRNA-derived fragments (tRFs): biogenesis, biological functions, and their role as potential biomarkers in human diseases.Journal of molecular medicine (Berlin, Germany) · 2022Review
- MicroRNAs: Harbingers and shapers of periodontal inflammation.Seminars in cell & developmental biology · 2022Review
- Identification and Characterization of the miRNAs and Cytokines in Response to Leishmania infantum Infection with Different Response to Treatment.Acta parasitologica · 2022Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundMicroRNAs (miRNAs) are a class of short non-coding RNA that play important roles in disease processes in animals and are present in a highly stable cell-free form in body fluids. Here, we examine the capacity of host and parasite miRNAs to serve as tissue or serum biomarkers of Schistosoma mansoni infection. METHODS/PRINCIPAL
findingsWe used Exiqon miRNA microarrays to profile miRNA expression in the livers of mice infected with S. mansoni at 7 weeks post-infection. Thirty-three mouse miRNAs were differentially expressed in infected compared to naïve mice (>2 fold change, p<0.05) including miR-199a-3p, miR-199a-5p, miR-214 and miR-21, which have previously been associated with liver fibrosis in other settings. Five of the mouse miRNAs were also significantly elevated in serum by twelve weeks post-infection. Sequencing of small RNAs from serum confirmed the presence of these miRNAs and further revealed eleven parasite-derived miRNAs that were detectable by eight weeks post infection. Analysis of host and parasite miRNA abundance by qRT-PCR was extended to serum of patients from low and high infection sites in Zimbabwe and Uganda. The host-derived miRNAs failed to distinguish uninfected from infected individuals. However, analysis of three of the parasite-derived miRNAs (miR-277, miR-3479-3p and bantam) could detect infected individuals from low and high infection intensity sites with specificity/sensitivity values of 89%/80% and 80%/90%, respectively.
conclusionsThis work identifies parasite-derived miRNAs as novel markers of S. mansoni infection in both mice and humans, with the potential to be used with existing techniques to improve S. mansoni diagnosis. In contrast, although host miRNAs are differentially expressed in the liver during infection their abundance levels in serum are variable in human patients and may be useful in cases of extreme pathology but likely hold limited value for detecting prevalence of infection.
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Registered trials
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