ArticlePloS one2014
Identification of suitable reference genes for gene expression normalization in qRT-PCR analysis in watermelon.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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98 citing papers in PubMed.
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- Genome-wide identification of HXK gene families in cucumber and watermelon: CsHXK1 and ClHXK6 play key roles in responding to multiple stresses.BMC plant biology · 2026Article
- The application of nitrate nitrogen fertilizer reduces the occurrence of bacterial fruit blotch of watermelon.Frontiers in plant science · 2026Article
- Identification and Validation of Reference Genes for Quantitative Real-Time PCR inPlants (Basel, Switzerland) · 2025Article
- qPCR-Based Reference Gene Validation inCurrent issues in molecular biology · 2025Article
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- Uncovering the genetic mechanism of rind color trait in watermelon using fine mapping and comparative transcriptomic analysis.Frontiers in plant science · 2025Article
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- Identification of the Optimal Quantitative RT-PCR Reference Gene for Paper Mulberry (Current issues in molecular biology · 2024Article
- Fine genetic mapping and transcriptomic analysis revealed major gene modulating the clear stripe margin pattern of watermelon peel.Frontiers in plant science · 2024Article
- Comprehensive Characterization of B-Box Zinc Finger Genes inPlants (Basel, Switzerland) · 2023Article
- Comparative bioinformatics analysis and abiotic stress responses of expansin proteins in Cucurbitaceae members: watermelon and melon.Protoplasma · 2023Article
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- Genetic mapping and molecular characterization of the delayed green geneFrontiers in plant science · 2023Article
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38 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Watermelon is one of the major Cucurbitaceae crops and the recent availability of genome sequence greatly facilitates the fundamental researches on it. Quantitative real-time reverse transcriptase PCR (qRT-PCR) is the preferred method for gene expression analyses, and using validated reference genes for normalization is crucial to ensure the accuracy of this method. However, a systematic validation of reference genes has not been conducted on watermelon. In this study, transcripts of 15 candidate reference genes were quantified in watermelon using qRT-PCR, and the stability of these genes was compared using geNorm and NormFinder. geNorm identified ClTUA and ClACT, ClEF1α and ClACT, and ClCAC and ClTUA as the best pairs of reference genes in watermelon organs and tissues under normal growth conditions, abiotic stress, and biotic stress, respectively. NormFinder identified ClYLS8, ClUBCP, and ClCAC as the best single reference genes under the above experimental conditions, respectively. ClYLS8 and ClPP2A were identified as the best reference genes across all samples. Two to nine reference genes were required for more reliable normalization depending on the experimental conditions. The widely used watermelon reference gene 18SrRNA was less stable than the other reference genes under the experimental conditions. Catalase family genes were identified in watermelon genome, and used to validate the reliability of the identified reference genes. ClCAT1and ClCAT2 were induced and upregulated in the first 24 h, whereas ClCAT3 was downregulated in the leaves under low temperature stress. However, the expression levels of these genes were significantly overestimated and misinterpreted when 18SrRNA was used as a reference gene. These results provide a good starting point for reference gene selection in qRT-PCR analyses involving watermelon.
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