ArticlePloS one2014
Characterisation of connexin expression and electrophysiological properties in stable clones of the HL-1 myocyte cell line.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
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- Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction.Acta pharmacologica Sinica · 2024Article
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- Synthetic recovery of impulse propagation in myocardial infarction via silicon carbide semiconductive nanowires.Nature communications · 2022Article
- Review
- Myocardial infarction affects Cx43 content of extracellular vesicles secreted by cardiomyocytes.Life science alliance · 2020Article
- MulticellularFrontiers in cardiovascular medicine · 2020Review
- Ranolazine-Mediated Attenuation of Mechanoelectric Feedback in Atrial Myocyte Monolayers.Frontiers in physiology · 2020Article
- Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting.Sensors (Basel, Switzerland) · 2019Article
- Experimental models of cardiac physiology and pathology.Heart failure reviews · 2019Review
- Development, in vitro validation and human application of a novel method to identify arrhythmia mechanisms: The stochastic trajectory analysis of ranked signals mapping method.Journal of cardiovascular electrophysiology · 2019Article
- New insights on the cardiac safety factor: Unraveling the relationship between conduction velocity and robustness of propagation.Journal of molecular and cellular cardiology · 2019Article
- Generation and primary characterization of iAM-1, a versatile new line of conditionally immortalized atrial myocytes with preserved cardiomyogenic differentiation capacity.Cardiovascular research · 2018Article
- Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line.Journal of molecular and cellular cardiology · 2018Article
- Rotor Tracking Using Phase of Electrograms Recorded During Atrial Fibrillation.Annals of biomedical engineering · 2017Article
- Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels.BMC cell biology · 2017Review
- Biology of the cardiac myocyte in heart disease.Molecular biology of the cell · 2016Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The HL-1 atrial line contains cells blocked at various developmental stages. To obtain homogeneous sub-clones and correlate changes in gene expression with functional alterations, individual clones were obtained and characterised for parameters involved in conduction and excitation-contraction coupling. Northern blots for mRNAs coding for connexins 40, 43 and 45 and calcium handling proteins (sodium/calcium exchanger, L- and T-type calcium channels, ryanodine receptor 2 and sarco-endoplasmic reticulum calcium ATPase 2) were performed. Connexin expression was further characterised by western blots and immunofluorescence. Inward currents were characterised by voltage clamp and conduction velocities measured using microelectrode arrays. The HL-1 clones had similar sodium and calcium inward currents with the exception of clone 2 which had a significantly smaller calcium current density. All the clones displayed homogenous propagation of electrical activity across the monolayer correlating with the levels of connexin expression. Conduction velocities were also more sensitive to inhibition of junctional coupling by carbenoxolone (∼ 80%) compared to inhibition of the sodium current by lidocaine (∼ 20%). Electrical coupling by gap junctions was the major determinant of conduction velocities in HL-1 cell lines. In summary we have isolated homogenous and stable HL-1 clones that display characteristics distinct from the heterogeneous properties of the original cell line.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.