ArticlePloS one2014
Syncytiotrophoblast vesicles show altered micro-RNA and haemoglobin content after ex-vivo perfusion of placentas with haemoglobin to mimic preeclampsia.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- An Investigation of Exosome Concentration and Exosomal microRNA (miR-155 and miR-222) Expression in Pregnant Women with Gestational Hypertension and Preeclampsia.International journal of women's health · 2022Article
- Large-scale transcriptome-wide profiling of microRNAs in human placenta and maternal plasma at early to mid gestation.RNA biology · 2021Article
- The Involvement of MicroRNAs in SARS-CoV-2 Infection Comorbid with HIV-Associated Preeclampsia.Current hypertension reports · 2021Review
- Exosomes as Biomarkers for Female Reproductive Diseases Diagnosis and Therapy.International journal of molecular sciences · 2021Review
- Vascular mechanisms and molecular targets in hypertensive pregnancy and preeclampsia.American journal of physiology. Heart and circulatory physiology · 2020Review
- Interrelationship Between Markers of Oxidative Stress, Inflammation and Hematological Parameters Among Preeclamptic Nigerian Women.Medical science monitor basic research · 2018Article
- MiR-616-3p modulates cell proliferation and migration through targeting tissue factor pathway inhibitor 2 in preeclampsia.Cell proliferation · 2018Article
- Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors.American journal of reproductive immunology (New York, N.Y. : 1989) · 2018Article
- Syncytiotrophoblast derived extracellular vesicles transfer functional placental miRNAs to primary human endothelial cells.Scientific reports · 2017Article
- Syncytiotrophoblast extracellular vesicles - Circulating biopsies reflecting placental health.Placenta · 2017Review
- Cross Talk between Adipose Tissue and Placenta in Obese and Gestational Diabetes Mellitus PregnanciesFrontiers in endocrinology · 2017Review
- Micro- and Nano-vesicles from First Trimester Human Placentae Carry Flt-1 and Levels Are Increased in Severe Preeclampsia.Frontiers in endocrinology · 2017Article
- Characterization of intravascular cellular activation in relationship to subclinical atherosclerosis in postmenopausal women.PloS one · 2017Article
- Placental extracellular vesicles and feto-maternal communication.Cold Spring Harbor perspectives in medicine · 2015Review
- Expression and significance of miR155 and vascular endothelial growth factor in placenta of rats with preeclampsia.International journal of clinical and experimental medicine · 2015Article
- A1M Ameliorates Preeclampsia-Like Symptoms in Placenta and Kidney Induced by Cell-Free Fetal Hemoglobin in Rabbit.PloS one · 2015Article
- Extracellular vesicles and reproduction-promotion of successful pregnancy.Cellular & molecular immunology · 2014Review
- Oxidative stress in preeclampsia and the role of free fetal hemoglobin.Frontiers in physiology · 2014Review
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Authors and funding
12 authors at 7 institutions in 6 countries.
Funding
Abstract
backgroundCell-free foetal haemoglobin (HbF) has been shown to play a role in the pathology of preeclampsia (PE). In the present study, we aimed to further characterize the harmful effects of extracellular free haemoglobin (Hb) on the placenta. In particular, we investigated whether cell-free Hb affects the release of placental syncytiotrophoblast vesicles (STBMs) and their micro-RNA content.
methodsThe dual ex-vivo perfusion system was used to perfuse isolated cotyledons from human placenta, with medium alone (control) or supplemented with cell-free Hb. Perfusion medium from the maternal side of the placenta was collected at the end of all perfusion phases. The STBMs were isolated using ultra-centrifugation, at 10,000×g and 150,000×g (referred to as 10K and 150K STBMs). The STBMs were characterized using the nanoparticle tracking analysis, identification of surface markers and transmission electron microscopy. RNA was extracted and nine different micro-RNAs, related to hypoxia, PE and Hb synthesis, were selected for analysis by quantitative PCR.
resultsAll micro-RNAs investigated were present in the STBMs. Mir-517a, mir-141 and mir-517b were down regulated after Hb perfusion in the 10K STBMs. Furthermore, Hb was shown to be carried by the STBMs.
conclusionThis study showed that Hb perfusion can alter the micro-RNA content of released STBMs. Of particular interest is the alteration of two placenta specific micro-RNAs; mir-517a and mir-517b. We have also seen that STBMs may function as carriers of Hb into the maternal circulation.
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