ArticlePloS one2014
Elevated exhaled nitric oxide in allergen-provoked asthma is associated with airway epithelial iNOS.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- A molecular systems architecture of asthma.Frontiers in immunology · 2026Pooled it
- Utility of low-dose oral aspirin challenges for diagnosis of aspirin-exacerbated respiratory disease.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2016Trial
- Cross-Cohort Transcriptomic Prioritization and Chemical-Provenance Assessment of Asthma Airway-Brushing Genes Overlapping HERB-AnnotatedInternational journal of molecular sciences · 2026Article
- The Role of Fractional Exhaled Nitric Oxide and Oscillometry in Pediatric Asthma.Respiratory care · 2025Review
- Clinical Utility of Central and Peripheral Airway Nitric Oxide in Children with Different Types of Allergic Asthma.Journal of asthma and allergy · 2025Article
- Association of alpha globin gene copy number with exhaled nitric oxide in a cross-sectional study of healthy Black adults.BMJ open respiratory research · 2023Article
- Combining nitric oxide and calcium sensing for the detection of endothelial dysfunction.Communications chemistry · 2023Review
- Preparation and Safety Evaluation ofEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Use of Fractional Exhaled Nitric Oxide to Guide the Treatment of Asthma: An Official American Thoracic Society Clinical Practice Guideline.American journal of respiratory and critical care medicine · 2021Article
- Type-2 airway inflammation in mild asthma patients with high blood eosinophils and high fractional exhaled nitric oxide.Clinical and translational science · 2021Observational
- A review of epigenetic changes in asthma: methylation and acetylation.Clinical epigenetics · 2021Review
- Nitric Oxide System and Bronchial Epithelium: More Than a Barrier.Frontiers in physiology · 2021Review
- Association Between FeNO, Total Blood IgE, Peripheral Blood Eosinophil and Inflammatory Cytokines in Partly Controlled Asthma.Journal of asthma and allergy · 2020Article
- Relationship between Particulate Matter (PMCanadian respiratory journal · 2020Article
- Bioenergetic Differences in the Airway Epithelium of LeanAntioxidants & redox signaling · 2019Article
- Severe Eosinophilic Asthma.Journal of clinical medicine · 2019Review
- Malar rash is a predictor of subclinical airway inflammation in patients with systemic lupus erythematosus: a pilot study.Clinical rheumatology · 2019Article
- Fractional Exhaled Nitric Oxide (FeNO) Combined with Pulmonary Function Parameters Shows Increased Sensitivity and Specificity for the Diagnosis of Cough Variant Asthma in Children.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Myeloid-derived suppressor cells (MDSCs) and mechanistic target of rapamycin (mTOR) signaling pathway interact through inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in asthma.American journal of translational research · 2019Article
- Design and synthesis of novel 1,3,5-triphenyl pyrazolines as potential anti-inflammatory agents through allosteric inhibition of protein kinase Czeta (PKCζ).MedChemComm · 2018Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFractional exhaled nitric oxide is elevated in allergen-provoked asthma. The cellular and molecular source of the elevated fractional exhaled nitric oxide is, however, uncertain.
objectiveTo investigate whether fractional exhaled nitric oxide is associated with increased airway epithelial inducible nitric oxide synthase (iNOS) in allergen-provoked asthma.
methodsFractional exhaled nitric oxide was measured in healthy controls (n = 14) and allergic asthmatics (n = 12), before and after bronchial provocation to birch pollen out of season. Bronchoscopy was performed before and 24 hours after allergen provocation. Bronchial biopsies and brush biopsies were processed for nitric oxide synthase activity staining with nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), iNOS immunostaining, or gene expression analysis of iNOS by real-time PCR. NADPH-d and iNOS staining were quantified using automated morphometric analysis.
resultsFractional exhaled nitric oxide and expression of iNOS mRNA were significantly higher in un-provoked asthmatics, compared to healthy controls. Allergic asthmatics exhibited a significant elevation of fractional exhaled nitric oxide after allergen provocation, as well as an accumulation of airway eosinophils. Moreover, nitric oxide synthase activity and expression of iNOS was significantly increased in the bronchial epithelium of asthmatics following allergen provocation. Fractional exhaled nitric oxide correlated with eosinophils and iNOS expression.
conclusionHigher fractional exhaled nitric oxide concentration among asthmatics is associated with elevated iNOS mRNA in the bronchial epithelium. Furthermore, our data demonstrates for the first time increased expression and activity of iNOS in the bronchial epithelium after allergen provocation, and thus provide a mechanistic explanation for elevated fractional exhaled nitric oxide in allergen-provoked asthma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.