ArticlePloS one2014
Genome sequencing of Listeria monocytogenes "Quargel" listeriosis outbreak strains reveals two different strains with distinct in vitro virulence potential.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 56 citations in OpenAlex.
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- Genomic characterization ofFrontiers in microbiology · 2024Article
- Human Listeriosis.Clinical microbiology reviews · 2023Review
- A Large-Scale Sequencing-Based Survey of Plasmids inFrontiers in microbiology · 2021Article
- LiSEQ - whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe.Microbial genomics · 2019Article
- The Novel Internalins InlP1 and InlP4 and the Internalin-Like Protein InlP3 Enhance the Pathogenicity ofFrontiers in microbiology · 2019Article
- Genomic analysis of a Raoultella ornithinolytica strain causing prosthetic joint infection in an immunocompetent patient.Scientific reports · 2018Article
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- Listeria monocytogenes Biofilms in the Wonderland of Food Industry.Pathogens (Basel, Switzerland) · 2017Review
- Listeria monocytogenes Strains Underrepresented during Selective Enrichment with an ISO Method Might Dominate during Passage through Simulated Gastric Fluid andApplied and environmental microbiology · 2016Article
- Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002-2012.Emerging infectious diseases · 2016Article
- Genome analysis of Listeria ivanovii strain G770 that caused a deadly aortic prosthesis infection.New microbes and new infections · 2016Article
- Processing-Dependent and Clonal Contamination Patterns of Listeria monocytogenes in the Cured Ham Food Chain Revealed by Genetic Analysis.Applied and environmental microbiology · 2016Article
- Prospective Whole-Genome Sequencing Enhances National Surveillance of Listeria monocytogenes.Journal of clinical microbiology · 2016Article
- Evolution and Diversity of Listeria monocytogenes from Clinical and Food Samples in Shanghai, China.Frontiers in microbiology · 2016Article
- Comparative Genomic Analysis of Two Serotype 1/2bFrontiers in nutrition · 2016Article
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- Influence of pH on bile sensitivity amongst various strains of Listeria monocytogenes under aerobic and anaerobic conditions.Journal of medical microbiology · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
A large listeriosis outbreak occurred in Austria, Germany and the Czech Republic in 2009 and 2010. The outbreak was traced back to a traditional Austrian curd cheese called "Quargel" which was contaminated with two distinct serovar 1/2a Listeria monocytogenes strains (QOC1 and QOC2). In this study we sequenced and analysed the genomes of both outbreak strains in order to investigate the extent of genetic diversity between the two strains belonging to MLST sequence types 398 (QOC2) and 403 (QOC1). Both genomes are highly similar, but also display distinct properties: The QOC1 genome is approximately 74 kbp larger than the QOC2 genome. In addition, the strains harbour 93 (QOC1) and 45 (QOC2) genes encoding strain-specific proteins. A 21 kbp region showing highest similarity to plasmid pLMIV encoding three putative internalins is integrated in the QOC1 genome. In contrast to QOC1, strain QOC2 harbours a vip homologue, which encodes a LPXTG surface protein involved in cell invasion. In accordance, in vitro virulence assays revealed distinct differences in invasion efficiency and intracellular proliferation within different cell types. The higher virulence potential of QOC1 in non-phagocytic cells may be explained by the presence of additional internalins in the pLMIV-like region, whereas the higher invasion capability of QOC2 into phagocytic cells may be due to the presence of a vip homologue. In addition, both strains show differences in stress-related gene content. Strain QOC1 encodes a so-called stress survival islet 1, whereas strain QOC2 harbours a homologue of the uncharacterized LMOf2365_0481 gene. Consistently, QOC1 shows higher resistance to acidic, alkaline and gastric stress. In conclusion, our results show that strain QOC1 and QOC2 are distinct and did not recently evolve from a common ancestor.
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