ArticlePloS one2014
Partial methylation at Am100 in 18S rRNA of baker's yeast reveals ribosome heterogeneity on the level of eukaryotic rRNA modification.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Article
- Comparative modeling reveals the molecular determinants of aneuploidy fitness cost in a wild yeast model.Cell genomics · 2024Article
- Comparative modeling reveals the molecular determinants of aneuploidy fitness cost in a wild yeast model.bioRxiv : the preprint server for biology · 2024Article
- Review
- The plant epitranscriptome: revisiting pseudouridine and 2'-O-methyl RNA modifications.Plant biotechnology journal · 2022Review
- Chemical Modifications of Ribosomal RNA.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mapping of the Chemical Modifications of rRNAs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Post-transcriptional diversity in riboproteins and RNAs in aging and cancer.Seminars in cancer biology · 2021Review
- Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing.Nature biotechnology · 2021Article
- Review
- The large repertoire of 2'-O-methylation guided by C/D snoRNAs on Trypanosoma brucei rRNA.RNA biology · 2020Article
- Holistic Optimization of Bioinformatic Analysis Pipeline for Detection and Quantification of 2'-O-Methylations in RNA by RiboMethSeq.Frontiers in genetics · 2020Article
- Small Non-Coding RNAs Derived From Eukaryotic Ribosomal RNA.Non-coding RNA · 2019Review
- 2'-O-Methylation of Ribosomal RNA: Towards an Epitranscriptomic Control of Translation?Biomolecules · 2018Review
- Mapping targets for small nucleolar RNAs in yeast.Wellcome open research · 2018Article
- Orchestrated positioning of post-transcriptional modifications at the branch point recognition region of U2 snRNA.RNA (New York, N.Y.) · 2018Article
- Identification of sites of 2'-O-methylation vulnerability in human ribosomal RNAs by systematic mapping.Scientific reports · 2017Article
- Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function.RNA biology · 2017Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosome heterogeneity is of increasing biological significance and several examples have been described for multicellular and single cells organisms. In here we show for the first time a variation in ribose methylation within the 18S rRNA of Saccharomyces cerevisiae. Using RNA-cleaving DNAzymes, we could specifically demonstrate that a significant amount of S. cerevisiae ribosomes are not methylated at 2'-O-ribose of A100 residue in the 18S rRNA. Furthermore, using LC-UV-MS/MS of a respective 18S rRNA fragment, we could not only corroborate the partial methylation at A100, but could also quantify the methylated versus non-methylated A100 residue. Here, we exhibit that only 68% of A100 in the 18S rRNA of S.cerevisiae are methylated at 2'-O ribose sugar. Polysomes also contain a similar heterogeneity for methylated Am100, which shows that 40S ribosome subunits with and without Am100 participate in translation. Introduction of a multicopy plasmid containing the corresponding methylation guide snoRNA gene SNR51 led to an increased A100 methylation, suggesting the cellular snR51 level to limit the extent of this modification. Partial rRNA modification demonstrates a new level of ribosome heterogeneity in eukaryotic cells that might have substantial impact on regulation and fine-tuning of the translation process.
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