ArticlePloS one2014
TALEN-mediated modification of the bovine genome for large-scale production of human serum albumin.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- A Pichia pastoris-based platform for large-scale production of functional recombinant human serum albumin with therapeutic efficacy.Bioprocess and biosystems engineering · 2026Article
- Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- High-efficiency production of human serum albumin in the posterior silk glands of transgenic silkworms, Bombyx mori L.PloS one · 2018Article
- Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer.Scientific reports · 2016Article
- Generation of TALE nickase-mediated gene-targeted cows expressing human serum albumin in mammary glands.Scientific reports · 2016Article
- Characterization of promoter of the tuberculosis-resistant gene intracellular pathogen resistance 1.Immunologic research · 2016Article
- Targeting Human α-Lactalbumin Gene Insertion into the Goat β-Lactoglobulin Locus by TALEN-Mediated Homologous Recombination.PloS one · 2016Article
- Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery.Transgenic research · 2015Article
- TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Genome engineering in cattle: recent technological advancements.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an initial step towards creating genetically modified cattle as a biopharming source of recombinant human serum albumin (rHSA), we report modification of the bovine albumin (bA) locus by transcription activator-like effector nuclease (TALEN)-stimulated homology-directed repair (HDR). Pedigreed bovine fibroblasts were co-transfected with TALENs and an 11.5-kb human serum albumin (HSA) minigene donor construct, designed to simultaneously disrupt and replace bovine serum albumin (BSA) expression with controlled rHSA expression in both the liver and the milk. Targeted integration of the HSA minigene was confirmed in transfected fibroblasts at a frequency of approximately 11% and transgenic bovine embryos were produced from targeted fibroblasts using somatic cell nuclear transfer (SCNT). The research delineated here lays the foundation for the future generation of transgenic rHSA cattle with the potential to provide a large-scale, reliable, and quality-controlled source of rHSA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.