ArticlePloS one2014
The direction of stretch-induced cell and stress fiber orientation depends on collagen matrix stress.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 87 citations in OpenAlex.
- Cyclic stretch inhibits cell invasion in 3D scaffolds.bioRxiv : the preprint server for biology · 2026Article
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- Orthogonal alignment of multilayered MC3T3-E1 cells induced by cyclic stretch.Biomechanics and modeling in mechanobiology · 2025Article
- Three-Dimensional Imaging of Human Myocardial Collagen Fibers Using Trypan Blue Staining.Sovremennye tekhnologii v meditsine · 2025Article
- Towards a more objective and high-throughput spheroid invasion assay quantification method.Scientific reports · 2024Article
- Towards a More Objective and High-throughput Spheroid Invasion Assay Quantification Method.bioRxiv : the preprint server for biology · 2024Article
- Can repetitive mechanical motion cause structural damage to axons?Frontiers in molecular neuroscience · 2024Review
- Cyclic Stretching Triggers Cell Orientation and Extracellular Matrix Remodeling in a Periodontal Ligament 3D In Vitro Model.Advanced healthcare materials · 2023Article
- Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli.Science advances · 2023Article
- Multicellular aligned bands disrupt global collective cell behavior.Acta biomaterialia · 2023Article
- A Statistical Mechanics Approach to Describe Cell Reorientation Under Stretch.Bulletin of mathematical biology · 2023Article
- Systems Biology Analysis of Temporal Dynamics That Govern Endothelial Response to Cyclic Stretch.Biomolecules · 2022Review
- Cell reorientation on a cyclically strained substrate.PNAS nexus · 2022Article
- A mechanistic motor-clutch model that explains cell shape dynamics to cyclic stretch.Molecular biology of the cell · 2022Article
- Editorial: Understanding molecular interactions that underpin vascular mechanobiology.APL bioengineering · 2021Article
- Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology.Communications biology · 2021Review
- Stress fibers of the aortic smooth muscle cells in tissues do not align with the principal strain direction during intraluminal pressurization.Biomechanics and modeling in mechanobiology · 2021Article
- Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis.Scientific reports · 2021Article
- NSCs Under Strain-Unraveling the Mechanoprotective Role of Differentiating Astrocytes in a Cyclically Stretched Coculture With Differentiating Neurons.Frontiers in cellular neuroscience · 2021Article
- Cutting the ovarian surface improves the responsiveness to exogenous hormonal treatment in aged mice.Reproductive medicine and biology · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell structure depends on both matrix strain and stiffness, but their interactive effects are poorly understood. We investigated the interactive roles of matrix properties and stretching patterns on cell structure by uniaxially stretching U2OS cells expressing GFP-actin on silicone rubber sheets supporting either a surface-adsorbed coating or thick hydrogel of type-I collagen. Cells and their actin stress fibers oriented perpendicular to the direction of cyclic stretch on collagen-coated sheets, but oriented parallel to the stretch direction on collagen gels. There was significant alignment parallel to the direction of a steady increase in stretch for cells on collagen gels, while cells on collagen-coated sheets did not align in any direction. The extent of alignment was dependent on both strain rate and duration. Stretch-induced alignment on collagen gels was blocked by the myosin light-chain kinase inhibitor ML7, but not by the Rho-kinase inhibitor Y27632. We propose that active orientation of the actin cytoskeleton perpendicular and parallel to direction of stretch on stiff and soft substrates, respectively, are responses that tend to maintain intracellular tension at an optimal level. Further, our results indicate that cells can align along directions of matrix stress without collagen fibril alignment, indicating that matrix stress can directly regulate cell morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.