ArticlePloS one2014
SAMHD1 restricts HIV-1 replication and regulates interferon production in mouse myeloid cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Protocol for generating human CAR-engineered macrophages by Vpx-containing lentivirus.STAR protocols · 2024Article
- Article
- Role of Intracellular Distribution of Feline and Bovine SAMHD1 Proteins in Lentiviral Restriction.Virologica Sinica · 2021Article
- A viral kinase counteracts in vivo restriction of murine cytomegalovirus by SAMHD1.Nature microbiology · 2019Article
- SAMHD1 suppresses innate immune responses to viral infections and inflammatory stimuli by inhibiting the NF-κB and interferon pathways.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- SAMHD1 inhibits epithelial cell transformation in vitro and affects leukemia development in xenograft mice.Cell cycle (Georgetown, Tex.) · 2018Article
- Article
- Influenza virus replication in macrophages: balancing protection and pathogenesis.The Journal of general virology · 2017Review
- A G1-like state allows HIV-1 to bypass SAMHD1 restriction in macrophages.The EMBO journal · 2017Article
- A Highly Active Isoform of Lentivirus Restriction Factor SAMHD1 in Mouse.The Journal of biological chemistry · 2017Article
- IFN-JCI insight · 2016Article
- TREX1 Knockdown Induces an Interferon Response to HIV that Delays Viral Infection in Humanized Mice.Cell reports · 2016Article
- SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load.Scientific reports · 2016Article
- Article
- Type I interferon: understanding its role in HIV pathogenesis and therapy.Current HIV/AIDS reports · 2015Review
- Didehydro-cortistatin A inhibits HIV-1 Tat mediated neuroinflammation and prevents potentiation of cocaine reward in Tat transgenic mice.Current HIV research · 2015Article
- Incorporating alternative splicing and mRNA editing into the genetic analysis of complex traits.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
SAMHD1 restricts the replication of HIV-1 and other retroviruses in human myeloid and resting CD4(+) T cells and that is counteracted in SIV and HIV-2 by the Vpx accessory protein. The protein is a phosphohydrolase that lowers the concentration of deoxynucleoside triphosphates (dNTP), blocking reverse transcription of the viral RNA genome. Polymorphisms in the gene encoding SAMHD1 are associated with Aicardi-Goutières Syndrome, a neurological disorder characterized by increased type-I interferon production. SAMHD1 is conserved in mammals but its role in restricting virus replication and controlling interferon production in non-primate species is not well understood. We show that SAMHD1 is catalytically active and expressed at high levels in mouse spleen, lymph nodes, thymus and lung. siRNA knock-down of SAMHD1 in bone marrow-derived macrophages increased their susceptibility to HIV-1 infection. shRNA knock-down of SAMHD1 in the murine monocytic cell-line RAW264.7 increased its susceptibility to HIV-1 and murine leukemia virus and increased the levels of the dNTP pool. In addition, SAMHD1 knock-down in RAW264.7 cells induced the production of type-I interferon and several interferon-stimulated genes, modeling the situation in Aicardi-Goutières Syndrome. Our findings suggest that the role of SAMHD1 in restricting viruses is conserved in the mouse. The RAW264.7 cell-line serves as a useful tool to study the antiviral and innate immune response functions of SAMHD1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.