Evidence map›Paper›PMID 24586748›Full record

ArticlePloS one2014

A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.

Jin-Bon Hong, Fu-Ju Chou, Amy T Ku, Hsiang-Hsuan Fan, Tung-Lung Lee, Yung-Hsin Huang, Tsung-Lin Yang, I-Chang Su, I-Shing Yu, Shu-Wha Lin and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Mining and engineering of activeSynthetic and systems biotechnology · 2027
    Article
  2. Article
  3. Article
  4. DNA Transposition at Work.Chemical reviews · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Jin-Bon HongGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan ; Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
Fu-Ju ChouGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan.
Amy T KuGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan.
Hsiang-Hsuan FanGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan.
Tung-Lung LeeDepartment of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
Yung-Hsin HuangDepartment of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
Tsung-Lin YangDepartment of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan ; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan.
I-Chang SuGraduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
I-Shing YuTransgenic Mouse Model Core Facility of the National Research Program for Genomic Medicine, National Taiwan University College of Medicine, Taipei, Taiwan ; Laboratory Animal Center, National Taiwan University College of Medicine, Taipei, Taiwan.
Shu-Wha LinDepartment of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan ; Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Chung-Liang ChienGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan ; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan ; Stem Cell Core Laboratory, National Taiwan University Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan ; Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan.
Hong-Nerng HoGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan ; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan ; Stem Cell Core Laboratory, National Taiwan University Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan ; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
You-Tzung ChenGraduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan ; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan ; Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan ; Stem Cell Core Laboratory, National Taiwan University Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan ; Genome and Systems Biology Program, National Taiwan University, Taipei, Taiwan.
National Taiwan University · TWNational Taiwan University Hospital · TW

Funding

Training Program in Translational Biology and Molecular MedicineT32GM088129 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI ROONEY, CLIONA M., VAN DEN VEYVER, IGNATIA B · 2010 to 2019
$3.0M
NIGMS NIH HHS T32 GM088129
6 · The paper itself

Abstract

PiggyBac is a prevalent transposon system used to deliver transgenes and functionally explore the mammalian untouched genomic territory. The important features of piggyBac transposon are the relatively low insertion site preference and the ability of seamless removal from genome, which allow its potential uses in functional genomics and regenerative medicine. Efforts to increase its transposition efficiency in mammals were made through engineering the corresponding transposase (PBase) codon usage to enhance its expression level and through screening for mutant PBase variants with increased enzyme activity. To improve the safety for its potential use in regenerative medicine applications, site-specific transposition was achieved by using engineered zinc finger- and Gal4-fused PBases. An excision-prone PBase variant has also been successfully developed. Here we describe the construction of a nucleolus-predominant PBase, NP-mPB, by adding a nucleolus-predominant (NP) signal peptide from HIV-1 TAT protein to a mammalian codon-optimized PBase (mPB). Although there is a predominant fraction of the NP-mPB-tGFP fusion proteins concentrated in the nucleoli, an insertion site preference toward nucleolar organizer regions is not detected. Instead a 3-4 fold increase in piggyBac transposition efficiency is reproducibly observed in mouse and human cells.

Indexed as

AnimalsCell Line, TumorCell NucleolusCells, CulturedCodonDNA Transposable ElementsGenomeHEK293 CellsHeLa CellsHIV-1HumansMammalsMiceMutagenesis, InsertionalTransgenesTransposasesCodonDNA Transposable ElementsTransposases

Identifiers

PMID24586748
PMCPMC3933532
OpenAlexW1987022017

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.