ArticlePloS one2014
RNA-seq analysis of transcriptome and glucosinolate metabolism in seeds and sprouts of broccoli (Brassica oleracea var. italic).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism.International journal of molecular sciences · 2023Article
- Targeted mutagenesis ofFrontiers in plant science · 2023Article
- BoPEP4, a C-Terminally Encoded Plant Elicitor Peptide from Broccoli, Plays a Role in Salinity Stress Tolerance.International journal of molecular sciences · 2022Article
- Enhancement of Glucosinolate Formation in Broccoli Sprouts by Hydrogen Peroxide Treatment.Foods (Basel, Switzerland) · 2022Article
- Insights into glucosinolate accumulation and metabolic pathways in Isatis indigotica Fort.BMC plant biology · 2022Article
- Transcriptome Analysis and Metabolic Profiling of Green and Red Mizuna (Foods (Basel, Switzerland) · 2020Article
- mGWAS Uncovers Gln-Glucosinolate Seed-Specific Interaction and its Role in Metabolic Homeostasis.Plant physiology · 2020Article
- Pre- and Post-harvest Factors Affecting Glucosinolate Content in Broccoli.Frontiers in nutrition · 2020Review
- Effect of Photoperiod on Chinese Kale (Frontiers in plant science · 2020Article
- Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused byInternational journal of molecular sciences · 2019Article
- Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts.BMC genomics · 2019Article
- Overexpressing the Myrosinase GeneFrontiers in plant science · 2019Article
- Transcriptome profiling of two contrasting ornamental cabbage (Brassica oleracea var. acephala) lines provides insights into purple and white inner leaf pigmentation.BMC genomics · 2018Article
- De Novo Transcriptome Sequencing of the Deep-Sea-Derived FungusInternational journal of molecular sciences · 2018Article
- Morpho-physiological and transcriptome profiling reveal novel zinc deficiency-responsive genes in rice.Functional & integrative genomics · 2017Article
- De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis.International journal of molecular sciences · 2017Article
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- Transcriptome Sequencing of Chemically Induced Aquilaria sinensis to Identify Genes Related to Agarwood Formation.PloS one · 2016Article
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4 authors.
Funding
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Abstract
backgroundBroccoli (Brassica oleracea var. italica), a member of Cruciferae, is an important vegetable containing high concentration of various nutritive and functional molecules especially the anticarcinogenic glucosinolates. The sprouts of broccoli contain 10-100 times higher level of glucoraphanin, the main contributor of the anticarcinogenesis, than the edible florets. Despite the broccoli sprouts' functional importance, currently available genetic and genomic tools for their studies are very limited, which greatly restricts the development of this functionally important vegetable.
resultsA total of ∼85 million 251 bp reads were obtained. After de novo assembly and searching the assembled transcripts against the Arabidopsis thaliana and NCBI nr databases, 19,441 top-hit transcripts were clustered as unigenes with an average length of 2,133 bp. These unigenes were classified according to their putative functional categories. Cluster analysis of total unigenes with similar expression patterns and differentially expressed unigenes among different tissues, as well as transcription factor analysis were performed. We identified 25 putative glucosinolate metabolism genes sharing 62.04-89.72% nucleotide sequence identity with the Arabidopsis orthologs. This established a broccoli glucosinolate metabolic pathway with high colinearity to Arabidopsis. Many of the biosynthetic and degradation genes showed higher expression after germination than in seeds; especially the expression of the myrosinase TGG2 was 20-130 times higher. These results along with the previous reports about these genes' studies in Arabidopsis and the glucosinolate concentration in broccoli sprouts indicate the breakdown products of glucosinolates may play important roles in the stage of broccoli seed germination and sprout development.
conclusionOur study provides the largest genetic resource of broccoli to date. These data will pave the way for further studies and genetic engineering of broccoli sprouts and will also provide new insight into the genomic research of this species and its relatives.
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