ArticlePloS one2014
Increased constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a decrease in microflora diversity may be indicators of ventilator-associated pneumonia: a prospective study in the respiratory tracts of neonates.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- The impact of meteorological factors on the respiratory pathogens of neonatal community-acquired pneumonia in Central China.BMC infectious diseases · 2025Article
- Characterization of the airway microbiome in preterm infants with bronchopulmonary dysplasia.Frontiers in cellular and infection microbiology · 2025Article
- Tongue coating microbiome composition reflects disease severity in patients with COVID-19 in Nanjing, China.Journal of oral microbiology · 2023Article
- Effect of invasive mechanical ventilation on the diversity of the pulmonary microbiota.Critical care (London, England) · 2022Review
- Oral dysbiosis and its linkage with SARS-CoV-2 infection.Microbiological research · 2022Article
- Metagenomic analysis reveals oropharyngeal microbiota alterations in patients with COVID-19.Signal transduction and targeted therapy · 2021Article
- Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients.Scientific reports · 2021Article
- A Genomic Approach To Identify Klebsiella pneumoniae and Acinetobacter baumannii Strains with Enhanced Competitive Fitness in the Lungs during Multistrain Pneumonia.Infection and immunity · 2019Article
- Microbiota in ICU, not only a gut problem.Intensive care medicine · 2019Article
- Patterns in the longitudinal oropharyngeal microbiome evolution related to ventilator-associated pneumonia.Antimicrobial resistance and infection control · 2019Observational
- Microbial diversity within the airway microbiome in chronic pediatric lung diseases.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Review
- Microbial investigations in throat swab and tracheal aspirate specimens are beneficial to predict the corresponding endotracheal tube biofilm flora among intubated neonates with ventilator-associated pneumonia.Experimental and therapeutic medicine · 2017Article
- Streptococcus sp. in neonatal endotracheal tube biofilms is associated with ventilator-associated pneumonia and enhanced biofilm formation of Pseudomonas aeruginosa PAO1.Scientific reports · 2017Article
- Dysbiosis in the intensive care unit: Microbiome science coming to the bedside.Journal of critical care · 2017Review
- The human microbiota: novel targets for hospital-acquired infections and antibiotic resistance.Annals of epidemiology · 2016Review
- Autoinducer-2 of Streptococcus mitis as a Target Molecule to Inhibit Pathogenic Multi-Species Biofilm Formation In Vitro and in an Endotracheal Intubation Rat Model.Frontiers in microbiology · 2016Article
- The microbiome and critical illness.The Lancet. Respiratory medicine · 2016Review
- Autoinducer-2 regulates Pseudomonas aeruginosa PAO1 biofilm formation and virulence production in a dose-dependent manner.BMC microbiology · 2015Article
- Molecular analysis of biofilms on the surface of neonatal endotracheal tubes based on 16S rRNA PCR-DGGE and species-specific PCR.International journal of clinical and experimental medicine · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ventilator-associated pneumonia (VAP) is a common complication and cause of death in neonates on mechanical ventilation. However, it is difficult to define the causes of VAP. To understand the causes of VAP, we undertook a prospective study based on the diversity of the microflora in VAP. The experimental group consisted of newborns who suffered from respiratory distress syndrome (RDS) and VAP, while the control group suffered from RDS without VAP. Sputa were collected within 1, 3, and 5 days of ventilation and were divided into six groups. DNA was extracted from the samples, and the 16S rDNA was PCR amplified, separated using denaturing gradient gel electrophoresis (DGGE), cloned and sequenced. The resulting sequences were compared using BLAST. The DGGE pictures were measured, and the richness, Shannon-Wiener index, and cluster maps were analyzed. No differences were found regarding the constituent ratio of any genus between the Non-VAP and VAP group within 1 day after intubation. After 1 to 3 days, the constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the VAP group were higher than those in the Non-VAP group, and the ratios of Serratia sp. and Achromobacter sp. were lower. After 3 to 5 days, the ratios of Klebsiella sp., Acinetobacter sp., Serratia sp., and Achromobacter sp. were lower than those in the Non-VAP group. The richness and Shannon-Wiener index of the Non-VAP group were higher than those of the VAP group from 1 to 3 days after intubation, while no differences were found within 1 day and from 3 to 5 days. We conclude that during the first three days of intubation, the microflora diversity in the lower respiratory tract was reduced due to VAP, and the greater constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the sputum may be indicators of VAP.
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