ArticleSmall (Weinheim an der Bergstrasse, Germany)2014
Ultraspecific and highly sensitive nucleic acid detection by integrating a DNA catalytic network with a label-free microcavity.
Article in Small (Weinheim an der Bergstrasse, Germany), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 62 citations in OpenAlex.
- High-Q WGM microcavity-based optofluidic sensor technologies for biological analysis.Biomicrofluidics · 2024Review
- Label-free, ultra-low detection limit DNA biosensor using high quality optical microcavity functionalized by DNA tetrahedral nanostructure probes.Nanophotonics (Berlin, Germany) · 2023Article
- A smart fluorescent biosensor for the highly sensitive detection of BRCA1 based on a 3D DNA walker and ESDR cascade amplification.RSC advances · 2019Article
- Advances of Optofluidic Microcavities for Microlasers and Biosensors.Micromachines · 2018Review
- Integrating a DNA Strand Displacement Reaction with a Whispering Gallery Mode Sensor for Label-Free Mercury (II) Ion Detection.Sensors (Basel, Switzerland) · 2016Article
- Applications of Optical Microcavity Resonators in Analytical Chemistry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
- A stochastic DNA walker that traverses a microparticle surface.Nature nanotechnology · 2016Article
- Whispering gallery mode sensors.Advances in optics and photonics · 2015Article
- Ratiometric detection of oligonucleotide stoichiometry on multifunctional gold nanoparticles by whispering gallery mode biosensing.The Analyst · 2015Article
- Whispering gallery mode resonators for rapid label-free biosensing in small volume droplets.Biosensors · 2015Article
- Label free detection of 5'hydroxymethylcytosine within CpG islands using optical sensors.Biosensors & bioelectronics · 2015Article
- Single-molecule nucleic acid interactions monitored on a label-free microcavity biosensor platform.Nature nanotechnology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Nucleic acid detection with label-free biosensors circumvents costly fluorophore functionalization steps associated with conventional assays by utilizing transducers of impressive ultimate detection limits. Despite this technological prowess, molecular recognition at a surface limits the biosensors' sensitivity, specificity, and reusability. It is therefore imperative to integrate novel molecular approaches with existing label-free transducers to overcome those limitations. Here, we demonstrate this concept by integrating a DNA strand displacement circuit with a micron-scale whispering gallery mode (WGM) microsphere biosensor. The integrated biosensor exhibits at least 25-fold improved nucleic acid sensitivity, and sets a new record for label-free microcavity biosensors by detecting 80 pM (32 fmol) of a 22nt oligomer; this improvement results from the catalytic behavior of the circuit. Furthermore, the integrated sensor exhibits extremely high specificity; single nucleotide variants yield 40- to 100-fold lower signal. Finally, the same physical sensor was demonstrated to alternatingly detect 2 different nucleic acid sequences through 5 cycles of detection, showcasing both its reusability and its versatility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.