ArticleThe Journal of biological chemistry2014
Structure and mechanism of soybean ATP sulfurylase and the committed step in plant sulfur assimilation.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Structure/function of ATP sulfurylase domain of human 3'-phosphoadenosine 5'-phosphosulfate synthase (hPAPSS).Biochemistry and biophysics reports · 2025Article
- Adaptive modifications in plant sulfur metabolism over evolutionary time.Journal of experimental botany · 2024Review
- Gene Identification, expression analysis and molecular docking of ATP sulfurylase in the selenization pathway of Cardamine hupingshanensis.BMC plant biology · 2022Article
- Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds.Scientific reports · 2020Article
- Article
- A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed (Brassica napus L.) germplasm.Plant biotechnology journal · 2020Article
- Isotopic Fractionation Associated With Sulfate Import and Activation byFrontiers in microbiology · 2020Article
- Integrated Analysis of Small RNA, Transcriptome and Degradome Sequencing Provides New Insights into Floral Development and Abscission in Yellow Lupine (International journal of molecular sciences · 2019Article
- Sulfation pathways from red to green.The Journal of biological chemistry · 2019Review
- Mechanism of Sulfate Activation Catalyzed by ATP Sulfurylase - Magnesium Inhibits the Activity.Computational and structural biotechnology journal · 2019Article
- Sulfur Partitioning between Glutathione and Protein Synthesis Determines Plant Growth.Plant physiology · 2018Article
- Impact of overexpression of cytosolic isoform of O-acetylserine sulfhydrylase on soybean nodulation and nodule metabolome.Scientific reports · 2018Article
- Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea.Scientific reports · 2017Article
- An integrated analysis of QTL mapping and RNA sequencing provides further insights and promising candidates for pod number variation in rapeseed (Brassica napus L.).BMC genomics · 2017Article
- Recapitulating the Structural Evolution of Redox Regulation in Adenosine 5'-Phosphosulfate Kinase from Cyanobacteria to Plants.The Journal of biological chemistry · 2015Article
- ATP-sulfurylase, sulfur-compounds, and plant stress tolerance.Frontiers in plant science · 2015Review
- Diversity and regulation of ATP sulfurylase in photosynthetic organisms.Frontiers in plant science · 2014Review
- Plastid-cytosol partitioning and integration of metabolic pathways for APS/PAPS biosynthesis in Arabidopsis thaliana.Frontiers in plant science · 2014Article
- Molecular mechanisms of regulation of sulfate assimilation: first steps on a long road.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes of the sulfur assimilation pathway are potential targets for improving nutrient content and environmental stress responses in plants. The committed step in this pathway is catalyzed by ATP sulfurylase, which synthesizes adenosine 5'-phosphosulfate (APS) from sulfate and ATP. To better understand the molecular basis of this energetically unfavorable reaction, the x-ray crystal structure of ATP sulfurylase isoform 1 from soybean (Glycine max ATP sulfurylase) in complex with APS was determined. This structure revealed several highly conserved substrate-binding motifs in the active site and a distinct dimerization interface compared with other ATP sulfurylases but was similar to mammalian 3'-phosphoadenosine 5'-phosphosulfate synthetase. Steady-state kinetic analysis of 20 G. max ATP sulfurylase point mutants suggests a reaction mechanism in which nucleophilic attack by sulfate on the α-phosphate of ATP involves transition state stabilization by Arg-248, Asn-249, His-255, and Arg-349. The structure and kinetic analysis suggest that ATP sulfurylase overcomes the energetic barrier of APS synthesis by distorting nucleotide structure and identifies critical residues for catalysis. Mutations that alter sulfate assimilation in Arabidopsis were mapped to the structure, which provides a molecular basis for understanding their effects on the sulfur assimilation pathway.
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