ArticleApplied and environmental microbiology2014
Top-down proteomic identification of Shiga toxin 2 subtypes from Shiga toxin-producing Escherichia coli by matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Proteomics as a promising biomarker in food authentication, quality and safety: A review.Food science & nutrition · 2022Review
- Proteomic Advances in Cereal and Vegetable Crops.Molecules (Basel, Switzerland) · 2021Review
- Article
- The Role of Proteomics in Bacterial Response to Antibiotics.Pharmaceuticals (Basel, Switzerland) · 2020Review
- PHANOTATE: a novel approach to gene identification in phage genomes.Bioinformatics (Oxford, England) · 2019Article
- Top-Down Proteomic Identification of Shiga Toxin 1 and 2 from PathogenicMicroorganisms · 2019Article
- Pathogen proteotyping: A rapidly developing application of mass spectrometry to address clinical concerns.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- Clinically-relevant Shiga toxin 2 subtypes from environmental Shiga toxin-producingClinical mass spectrometry (Del Mar, Calif.) · 2019Article
- Top-down and middle-down proteomic analysis of Shiga toxin using MALDI-TOF-TOF mass spectrometry.MethodsX · 2019Article
- Cellular recovery from exposure to sub-optimal concentrations of AB toxins that inhibit protein synthesis.Scientific reports · 2018Article
- Article
- Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria.Proteomics. Clinical applications · 2016Review
- Personalized Proteomics: The Future of Precision Medicine.Proteomes · 2016Article
- A peptide identification-free, genome sequence-independent shotgun proteomics workflow for strain-level bacterial differentiation.Scientific reports · 2015Article
- Shiga toxin 2 subtypes of enterohemorrhagic E. coli O157:H- E32511 analyzed by RT-qPCR and top-down proteomics using MALDI-TOF-TOF-MS.Journal of the American Society for Mass Spectrometry · 2015Article
- Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry: do we need guidance for data interpretation?Journal of clinical microbiology · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed 26 Shiga toxin-producing Escherichia coli (STEC) strains for Shiga toxin 2 (Stx2) production using matrix-assisted laser desorption ionization (MALDI)-tandem time of flight (TOF-TOF) tandem mass spectrometry (MS/MS) and top-down proteomic analysis. STEC strains were induced to overexpress Stx2 by overnight culturing on solid agar supplemented with either ciprofloxacin or mitomycin C. Harvested cells were lysed by bead beating, and unfractionated bacterial cell lysates were ionized by MALDI. The A2 fragment of the A subunit and the mature B subunit of Stx2 were analyzed by MS/MS. Sequence-specific fragment ions were used to identify amino acid subtypes of Stx2 using top-down proteomic analysis using software developed in-house at the U.S. Department of Agriculture (USDA). Stx2 subtypes (a, c, d, f, and g) were identified on the basis of the mass of the A2 fragment and the B subunit as well as from their sequence-specific fragment ions by MS/MS (postsource decay). Top-down proteomic identification was in agreement with DNA sequencing of the full Stx2 operon (stx2) for all strains. Top-down results were also compared to a bioassay using a Vero-d2EGFP cell line. Our results suggest that top-down proteomic identification is a rapid, highly specific technique for distinguishing Stx2 subtypes.
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