ArticleApplied and environmental microbiology2014
Adaptation of Escherichia coli to elevated sodium concentrations increases cation tolerance and enables greater lactic acid production.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
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- Integration of (S)-2,3-oxidosqualene enables E. coli to become Iron Man E. coli with improved overall tolerance.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products.Microbial cell factories · 2023Article
- Carotenoids synthesis affects the salt tolerance mechanism ofFrontiers in microbiology · 2023Article
- Extended spectrum beta-lactamase-producingOne health (Amsterdam, Netherlands) · 2021Article
- Review
- Effects of subzero saline chilling on broiler chilling efficiency, meat quality, and microbial safety.Poultry science · 2020Article
- A bioluminescent reporter for the halophilic archaeon Haloferax volcanii.Extremophiles : life under extreme conditions · 2020Article
- The Effect of Population Bottleneck Size and Selective Regime on Genetic Diversity and Evolvability in Bacteria.Genome biology and evolution · 2019Article
- Enhanced 2-keto-L-gulonic acid production by applying L-sorbose-tolerant helper strain in the co-culture system.AMB Express · 2018Article
- Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution.Microbial cell factories · 2017Article
- Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli.Microbial cell factories · 2017Article
- Efficient fermentative production of polymer-grade D-lactate by an engineered alkaliphilic Bacillus sp. strain under non-sterile conditions.Microbial cell factories · 2016Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Adaptive evolution was employed to generate sodium (Na(+))-tolerant mutants of Escherichia coli MG1655. Four mutants with elevated sodium tolerance, designated ALS1184, ALS1185, ALS1186, and ALS1187, were independently isolated after 73 days of serial transfer in medium containing progressively greater Na(+) concentrations. The isolates also showed increased tolerance of K(+), although this cation was not used for selective pressure. None of the adapted mutants showed increased tolerance to the nonionic osmolyte sucrose. Several physiological parameters of E. coli MG1655 and ALS1187, the isolate with the greatest Na(+) tolerance, were calculated and compared using glucose-limited chemostats. Genome sequencing showed that the ALS1187 isolate contained mutations in five genes, emrR, hfq, kil, rpsG, and sspA, all of which could potentially affect the ability of E. coli to tolerate Na(+). Two of these genes, hfq and sspA, are known to be involved in global regulatory processes that help cells endure a variety of cellular stresses. Pyruvate formate lyase knockouts were constructed in strains MG1655 and ALS1187 to determine whether increased Na(+) tolerance afforded increased anaerobic generation of lactate. In fed-batch fermentations, E. coli ALS1187 pflB generated 76.2 g/liter lactate compared to MG1655 pflB, which generated only 56.3 g/liter lactate.
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