ArticleCell cycle (Georgetown, Tex.)2014
Nuclear accumulation of cyclin D1 following long-term fractionated exposures to low-dose ionizing radiation in normal human diploid cells.
Article in Cell cycle (Georgetown, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Knockdown of hCINAP sensitizes colorectal cancer cells to ionizing radiation.Cell cycle (Georgetown, Tex.) · 2024Article
- Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts.Journal of radiation research · 2022Article
- Ionizing Radiation Protein Biomarkers in Normal Tissue and Their Correlation to Radiosensitivity: A Systematic Review.Journal of personalized medicine · 2021Review
- Article
- Xeroderma Pigmentosum Group D (XPD) Inhibits the Proliferation Cycle of Vascular Smooth Muscle Cell (VSMC) by Activating Glycogen Synthase Kinase 3β (GSK3β).Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Could a plant derived protein potentiate the anticancer effects of a stem cell in brain cancer?Oncotarget · 2018Article
- Evaluation of a Small Animal Irradiation System for Animal Experiments Using EBT3 Model GAFCHROMIC™ Film.The Kobe journal of medical sciences · 2018Article
- A comparison of radiation-induced mitochondrial damage between neural progenitor stem cells and differentiated cells.Cell cycle (Georgetown, Tex.) · 2017Article
- ATM-mediated mitochondrial damage response triggered by nuclear DNA damage in normal human lung fibroblasts.Cell cycle (Georgetown, Tex.) · 2017Article
- Mitochondrial reactive oxygen species-mediated genomic instability in low-dose irradiated human cells through nuclear retention of cyclin D1.Cell cycle (Georgetown, Tex.) · 2016Review
- Article
- Severe mitochondrial damage associated with low-dose radiation sensitivity in ATM- and NBS1-deficient cells.Cell cycle (Georgetown, Tex.) · 2016Article
- DNA damage signaling guards against perturbation of cyclin D1 expression triggered by low-dose long-term fractionated radiation.Oncogenesis · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclin D1 is a mitogenic sensor that responds to growth signals from the extracellular environment and regulates the G 1-to-S cell cycle transition. When cells are acutely irradiated with a single dose of 10 Gy, cyclin D1 is degraded, causing cell cycle arrest at the G 1/S checkpoint. In contrast, cyclin D1 accumulates in human tumor cells that are exposed to long-term fractionated radiation (0.5 Gy/fraction of X-rays). In this study we investigated the effect of fractionated low-dose radiation exposure on cyclin D1 localization in 3 strains of normal human fibroblasts. To specifically examine the nuclear accumulation of cyclin D1, cells were treated with a hypotonic buffer containing detergent to remove cytoplasmic cyclin D1. Proliferating cell nuclear antigen (PCNA) immunofluorescence was used to identify cells in S phase. With this approach, we observed S-phase nuclear retention of cyclin D1 following low-dose fractionated exposures, and found that cyclin D1 nuclear retention increased with exposure time. Cells that retained nuclear cyclin D1 were more likely to have micronuclei than non-retaining cells, indicating that the accumulation of nuclear cyclin D1 was associated with genomic instability. Moreover, inhibition of the v-akt murine thymoma viral oncogene homolog (AKT) pathway facilitated cyclin D1 degradation and eliminated cyclin D1 nuclear retention in cells exposed to fractionated radiation. Thus, cyclin D1 may represent a useful marker for monitoring long-term effects associated with exposure to low levels of radiation.
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