ArticleAtherosclerosis2014
Effect of shear stress on water and LDL transport through cultured endothelial cell monolayers.
Article in Atherosclerosis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Vascular mechanical forces and vascular diseases.Journal of advanced research · 2026Review
- Low or oscillatory shear stress and endothelial permeability in atherosclerosis.Frontiers in physiology · 2024Review
- Modelling lung diffusion-perfusion limitation in mechanically ventilated SARS-CoV-2 patients.Frontiers in physiology · 2024Article
- Biomechanical determinants of endothelial permeability assessed in standard and modified hollow-fibre bioreactors.Journal of the Royal Society, Interface · 2023Article
- NO Synthesis but Not Apoptosis, Mitosis or Inflammation Can Explain Correlations between Flow Directionality and Paracellular Permeability of Cultured Endothelium.International journal of molecular sciences · 2022Article
- Pathophysiology of Atherosclerosis.International journal of molecular sciences · 2022Review
- Effects of stent shape on focal hemodynamics in intracranial atherosclerotic stenosis: A simulation study with computational fluid dynamics modeling.Frontiers in neurology · 2022Article
- Role of the Air-Blood Barrier Phenotype in Lung Oxygen Uptake and Control of Extravascular Water.Frontiers in physiology · 2022Article
- Endocytosis in the adaptation to cellular stress.Cell stress · 2020Review
- Overview of the Assessment of Endothelial Function in Humans.Frontiers in medicine · 2020Review
- Atrial septal defect and patent foramen ovale: early and long-term effects on endothelial function after percutaneous occlusion procedure.Heart and vessels · 2019Article
- Permeability of Epithelial/Endothelial Barriers in Transwells and Microfluidic Bilayer Devices.Micromachines · 2019Article
- Hydraulic conductivity and low-density lipoprotein transport of the venous graft wall in an arterial bypass.Biomedical engineering online · 2019Article
- Fibroblast Growth Factor-2 Binding to Heparan Sulfate Proteoglycans Varies with Shear Stress in Flow-Adapted Cells.Annals of biomedical engineering · 2019Article
- Stress phase angle regulates differentiation of human adipose-derived stem cells toward endothelial phenotype.Progress in biomaterials · 2018Article
- Effect of flow on targeting and penetration of angiopep-decorated nanoparticles in a microfluidic model blood-brain barrier.PloS one · 2018Article
- The Effect of Fluid Shear Stress on the In Vitro Release Kinetics of Sirolimus from PLGA Films.Polymers · 2017Article
- Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow.American journal of physiology. Heart and circulatory physiology · 2017Article
- Using cultured endothelial cells to study endothelial barrier dysfunction: Challenges and opportunities.American journal of physiology. Lung cellular and molecular physiology · 2016Review
- Hydraulic Conductivity of Smooth Muscle Cell-Initiated Arterial Cocultures.Annals of biomedical engineering · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Previous animal experiments have shown that the transport of LDL into arterial walls is shear stress dependent. However, little work has probed shear effects on LDL transport in vitro where conditions are well defined and mechanisms are more easily explored. Therefore, we measured shear induced water and LDL fluxes across cultured bovine aortic endothelial (BAEC) monolayers in vitro and developed a three-pore model to describe the transport dynamics. Cell apoptosis was quantified by TdT-mediated dUTP nick end labeling (TUNEL) assay. We also examined the role of nitric oxide (NO) in shear induced water and LDL fluxes by incubating BAEC monolayers with an NO synthase inhibitor, NG-monomethyl-L-arginine (L-NMMA). Our results show that direct exposure of endothelial monolayers to 12 dyn/cm2 shear stress for 3 h elicited a 2.37-fold increase in water flux (Jv), a 3.00-fold increase in LDL permeability (Pe), a 1.32-fold increase in LDL uptake, and a 1.68-fold increase in apoptotic rate. L-NMMA treatment of BAEC monolayers blocked shear induced Jv response, but had no significant effect on shear responses of Pe and cell apoptosis. A long time shear exposure (12 h) of endothelial monolayers reduced Pe and apoptotic rate close to the baseline. These results suggest that an acute change in shear stress from a static baseline state induces increases in water flux that are mediated by an NO dependent mechanism. On the other hand, the permeability of endothelial monolayers to LDL is enhanced by a short term-shear application and reduced nearly to the baseline level by a longer time shear exposure, positively correlated to the leaky junctions forming around apoptotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.