ArticleCurrent biology : CB2014
Dependency of the spindle assembly checkpoint on Cdk1 renders the anaphase transition irreversible.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 67 citations in OpenAlex.
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- ALKBH5 controls the meiosis-coupled mRNA clearance in oocytes by removing the NNature communications · 2023Article
- On the assembly of the mitotic spindle, bistability and hysteresis.Cellular and molecular life sciences : CMLS · 2023Article
- Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.EMBO reports · 2022Article
- A modular approach for modeling the cell cycle based on functional response curves.PLoS computational biology · 2021Article
- A prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes.Nature communications · 2021Article
- Dynamic bistable switches enhance robustness and accuracy of cell cycle transitions.PLoS computational biology · 2021Article
- Network mechanisms and dysfunction within an integrated computational model of progression through mitosis in the human cell cycle.PLoS computational biology · 2020Article
- Recent advances in understanding the role of Cdk1 in the Spindle Assembly Checkpoint.F1000Research · 2020Review
- Cyclin-dependent kinase 1 disruption inhibits angiogenesis by inducing cell cycle arrest and apoptosis.Experimental and therapeutic medicine · 2019Article
- Article
- The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.PLoS genetics · 2019Article
- Cycling through mammalian meiosis: B-type cyclins in oocytes.Cell cycle (Georgetown, Tex.) · 2019Article
- Cyclin B3 promotes anaphase I onset in oocyte meiosis.The Journal of cell biology · 2019Article
- CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.The Journal of cell biology · 2019Article
- Genome Stability during Cell Proliferation: A Systems Analysis of the Molecular Mechanisms Controlling Progression through the Eukaryotic Cell Cycle.Current opinion in systems biology · 2018Article
- Kinase and Phosphatase Cross-Talk at the Kinetochore.Frontiers in cell and developmental biology · 2018Review
- Cell-cycle transitions: a common role for stoichiometric inhibitors.Molecular biology of the cell · 2017Article
- Interlinked bistable mechanisms generate robust mitotic transitions.Cell cycle (Georgetown, Tex.) · 2017Review
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8 authors at 2 institutions in 2 countries.
Funding
Abstract
Activation of anaphase-promoting complex/cyclosome (APC/C(Cdc20)) by Cdc20 is delayed by the spindle assembly checkpoint (SAC). When all kinetochores come under tension, the SAC is turned off and APC/C(Cdc20) degrades cyclin B and securin, which activates separase [1]. The latter then cleaves cohesin holding sister chromatids together [2]. Because cohesin cleavage also destroys the tension responsible for turning off the SAC, cells must possess a mechanism to prevent SAC reactivation during anaphase, which could be conferred by a dependence of the SAC on Cdk1 [3-5]. To test this, we analyzed mouse oocytes and embryos expressing nondegradable cyclin B together with a Cdk1-resistant form of separase. After biorientation and SAC inactivation, APC/C(Cdc20) activates separase but the resulting loss of (some) cohesion is accompanied by SAC reactivation and APC/C(Cdc20) inhibition, which aborts the process of further securin degradation. Cyclin B is therefore the only APC/C(Cdc20) substrate whose degradation at the onset of anaphase is necessary to prevent SAC reactivation. The mutual activation of tension sensitive SAC and Cdk1 creates a bistable system that ensures complete activation of separase and total downregulation of Cdk1 when all chromosomes have bioriented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.