ArticleArthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association2014
Properties of biologic scaffolds and their response to mesenchymal stem cells.
Article in Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies.Clinical oral investigations · 2020Pooled it
- In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.International journal of molecular sciences · 2025Article
- Mesenchymal stem cells in PRP and PRF containing poly(3-caprolactone)/gelatin Scaffold: a comparative in-vitro study.Cell and tissue banking · 2024Article
- Effect of autologous lyophilized platelet‑rich fibrin on the reconstruction of osteochondral defects in rabbits.Experimental and therapeutic medicine · 2023Article
- Effect of Blood Gel Derivatives on Wound Healing in Mouse Injured Tissue Models.Gels (Basel, Switzerland) · 2023Article
- Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla.Dentistry journal · 2023Article
- Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration.Bioengineering (Basel, Switzerland) · 2023Review
- Article
- Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside.International journal of molecular sciences · 2019Review
- Therapeutic Effects of Umbilical Cord Blood-Derived Mesenchymal Stem Cells Combined with Polydeoxyribonucleotides on Full-Thickness Rotator Cuff Tendon Tear in a Rabbit Model.Cell transplantation · 2018Article
- Regenerative Engineering of the Rotator Cuff of the Shoulder.ACS biomaterials science & engineering · 2018Article
- The Role of Biologic Therapy in Rotator Cuff Tears and Repairs.Current reviews in musculoskeletal medicine · 2018Review
- Micro-Nanostructures of Cellulose-Collagen for Critical Sized Bone Defect Healing.Macromolecular bioscience · 2018Article
- Platelet Rich Plasma and Culture Configuration Affect the Matrix Forming Phenotype of Bone Marrow Stromal Cells.Tissue engineering and regenerative medicine · 2017Article
- Advances in biologic augmentation for rotator cuff repair.Annals of the New York Academy of Sciences · 2016Review
- Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study.BMC complementary and alternative medicine · 2016Article
- Stem cell procedures in arthroscopic surgery.European journal of medical research · 2016Review
- AAOS Research Symposium Updates and Consensus: Biologic Treatment of Orthopaedic Injuries.The Journal of the American Academy of Orthopaedic Surgeons · 2016Review
- Stem cell therapy in the management of shoulder rotator cuff disorders.World journal of stem cells · 2015Review
- Bone marrow derived stem cells in joint and bone diseases: a concise review.International orthopaedics · 2014Review
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to examine, in vitro, the cellular response of human mesenchymal stem cells (MSCs) to sample types of commercially available scaffolds in comparison with control, native tendon tissue (fresh-frozen rotator cuff tendon allograft).
methodsMSCs were defined by (1) colony-forming potential; (2) ability to differentiate into tendon, cartilage, bone, and fat tissue; and (3) fluorescence-activated cell sorting analysis (CD73, CD90, CD45). Samples were taken from fresh-frozen human rotator cuff tendon (allograft), human highly cross-linked collagen membrane (Arthroflex; LifeNet Health, Virginia Beach, VA), porcine non-cross-linked collagen membrane (Mucograft; Geistlich Pharma, Lucerne, Switzerland), a human platelet-rich fibrin matrix (PRF-M), and a fibrin matrix based on platelet-rich plasma (ViscoGel; Arthrex, Naples, FL). Cells were counted for adhesion (24 hours), thymidine assay for cell proliferation (96 hours), and live/dead stain for viability (168 hours). Histologic analysis was performed after 21 days, and the unloaded scaffolds were scanned with electron microscopy.
resultsMSCs were successfully differentiated into all cell lines. A significantly greater number of cells adhered to both the non-cross-linked porcine collagen scaffold and PRF-M. Cell activity (proliferation) was significantly higher in the non-cross-linked porcine collagen scaffold compared with PRF-M and fibrin matrix based on platelet-rich plasma. There were no significant differences found in the results of the live/dead assay.
conclusionsSignificant differences in the response of human MSCs to biologic scaffolds existed. MSC adhesion, proliferation, and scaffold morphology evaluated by histologic analysis and electron microscopy varied throughout the evaluated types of scaffolds. Non-cross-linked porcine collagen scaffolds showed superior results for cell adhesion and proliferation, as well as on histologic evaluation. CLINICAL RELEVANCE: This study enables the clinician and scientist to choose scaffold materials according to their specific interaction with MSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.