ArticleBMC genomics2014
Characterisation of Caenorhabditis elegans sperm transcriptome and proteome.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 59 citations in OpenAlex.
- SPE-55 and VHA-18 are essential for FB-MO complex assembly and transport during spermatogenesis in C. elegans.Nature communications · 2026Article
- TENT-5 polyadenylates and regulates male-specific transcripts in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2026Article
- The novel HRD motif kinase SPE-60 is required for sperm development and motility in Caenorhabditis elegans.Scientific reports · 2025Article
- A genetic framework for RNAi inheritance in Caenorhabditis elegans.EMBO reports · 2025Article
- RMD-3 and RMD-6 identified as new components of the MSP fibrous body complex inmicroPublication biology · 2025Article
- ADBP-1 regulates ADR-2 nuclear localization to control editing substrate selection.Nucleic acids research · 2024Article
- Single-tissue proteomics inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- hsp-90 and unc-45 depletion induce characteristic transcriptional signatures in coexpression cliques of C. elegans.Scientific reports · 2021Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Widespread misregulation of inter-species hybrid transcriptomes due to sex-specific and sex-chromosome regulatory evolution.PLoS genetics · 2021Article
- Paternal mitochondria from anmicroPublication biology · 2021Article
- Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans.Scientific reports · 2020Article
- Post-ejaculatory modifications to sperm (PEMS).Biological reviews of the Cambridge Philosophical Society · 2020Review
- Functionally non-redundant paralogs spe-47 and spe-50 encode FB-MO associated proteins and interact with him-8.PloS one · 2020Article
- Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.PLoS genetics · 2019Article
- A RNA-Seq Analysis to Describe the Boar Sperm Transcriptome and Its Seasonal Changes.Frontiers in genetics · 2019Article
- Proteomic and evolutionary analyses of sperm activation identify uncharacterized genes in Caenorhabditis nematodes.BMC genomics · 2018Article
- Caenorhabditis elegans sperm membrane protein interactome.Biology of reproduction · 2018Article
- Early experiences mediate distinct adult gene expression and reproductive programs in Caenorhabditis elegans.PLoS genetics · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough sperm is transcriptionally and translationally quiescent, complex populations of RNAs, including mRNAs and non-coding RNAs, exist in sperm. Previous microarray analysis of germ cell mutants identified hundreds of sperm genes in Caenorhabditis elegans. To take a more comprehensive view on C. elegans sperm genes, here, we isolate highly pure sperm cells and employ high-throughput technologies to obtain sperm transcriptome and proteome.
resultsFirst, sperm transcriptome consists of considerable amounts of non-coding RNAs, many of which have not been annotated and may play functional roles during spermatogenesis. Second, apart from kinases/phosphatases as previously reported, ion binding proteins are also enriched in sperm, underlying the crucial roles of intracellular ions in post-translational regulation in sperm. Third, while the majority of sperm genes/proteins have low abundance, a small number of sperm genes/proteins are hugely enriched in sperm, implying that sperm only rely on a small set of proteins for post-translational regulation. Lastly, by extensive RNAi screening of sperm enriched genes, we identified a few genes that control fertility. Our further analysis reveals a tight correlation between sperm transcriptome and sperm small RNAome, suggesting that the endogenous siRNAs strongly repress sperm genes. This leads to an idea that the inefficient RNAi screening of sperm genes, a phenomenon currently with unknown causes, might result from the competition between the endogenous RNAi pathway and the exogenous RNAi pathway.
conclusionsTogether, the obtained sperm transcriptome and proteome serve as valuable resources to systematically study spermatogenesis in C. elegans.
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