Evidence map›Paper›PMID 24578871›Full record

ArticleCentral European journal of urology2011

Schwann cells - a new hope in tissue engineered urinary bladder innervation. A method of cell isolation.

Jan Adamowicz, Tomasz Drewa, Jakub Tworkiewicz, Tomasz Kloskowski, Maciej Nowacki, Marta Pokrywczyńska

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In one paragraph

Article in Central European journal of urology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jan AdamowiczTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.
Tomasz DrewaTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland ; Department of Urology, Oncology Centre, prof. Franciszek Łukaszczyk Memorial Hospital, Bydgoszcz, Poland.
Jakub TworkiewiczTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland ; Department of Urology, Oncology Centre, prof. Franciszek Łukaszczyk Memorial Hospital, Bydgoszcz, Poland.
Tomasz KloskowskiTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.
Maciej NowackiTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.
Marta PokrywczyńskaTissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.
Nicolaus Copernicus University · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThere are not any effective method to induce the innervation of urinary bladder wall graft after augmentation. Neurons from urinary bladder wall and omentium can not elongate and branch in graft because of lack of neurotrophic factors. The best source of these neurotrophic factors are Schwann cells which can be transplanted into urinary bladder wall graft. To transplant Schwann cells the proper amount of cells is needed which can be only obtain during in vitro Schwann cell cultivation. We introduce the results of Schwann cell isolation and in vitro cultivation. MATERIALS AND

methods33 Wistar rats, males (350 gr.) were used in this study. Animal were divited into two groups (n = 15). Cell cultures were established in both groups on 5, 6, 7, 8 nad 9 day after nerve injury. In first group the digestion time with colagenase and trypsyne was 2.5 h and in second one 3.5 h.

resultsA larger number of cells were isolated from the degenerated sciatic nerve. Colonies of cells that morphologically resembled Schwann cells were visible by light microscopy on the second day of in vitro cultivation. Homogeneity of the primary cultures increased in the last day of cultivation to 60%.

conclusionsSchwann cells isolation from predegenerated peripheral nerve is effective and can delivered require amount of cells for transplantation to urinary bladder graft.

Indexed as

cell isolationinnervationSchwann cellstissue engineeringurinary bladder

Identifiers

PMID24578871
PMCPMC3921712
OpenAlexW2288412740

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.