Evidence map›Paper›PMID 24578380›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2014

Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA.

Robert D Bell, Xiaochun Long, Mingyan Lin, Jan H Bergmann, Vivek Nanda, Sarah L Cowan, Qian Zhou, Yu Han, David L Spector, Deyou Zheng and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
163citing papers in PubMed, 2 pooled it
9.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

163 citing papers in PubMed, 2 syntheses or guidelines pooled it, 288 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  7. Article
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  9. Long Non-Coding RNA Function in Smooth Muscle Cell Plasticity and Atherosclerosis.Arteriosclerosis, thrombosis, and vascular biology · 2025
    Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Long Noncoding RNA MALAT1: Salt-Sensitive Hypertension.International journal of molecular sciences · 2024
    Review
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article

103 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Robert D BellFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Xiaochun LongFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Mingyan LinFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Jan H BergmannFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Vivek NandaFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Sarah L CowanFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Qian ZhouFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Yu HanFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
David L SpectorFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Deyou ZhengFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.).
Joseph M MianoFrom the Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY (R.D.B., X.L., V.N., S.L.C., Q.Z., Y.H., J.M.M.); Department of Genetics (M.L., D.Z.) and Departments of Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (J.H.B., D.L.S.). j.m.miano@rochester.edu deyou.zheng@einstein.yu.edu.
Albert Einstein College of Medicine · US

Funding

VIRAL TRANSACTIVATIONP01CA013106 · NCI · COLD SPRING HARBOR LABORATORY · PI William Richard McCombie · 1985 to 2026
$116.8M
Novel Role of MAPK14 in Regulation of VSMC Contractile PhenotypeR01HL122686 · NHLBI · AUGUSTA UNIVERSITY · PI LONG, XIAOCHUN · 2014 to 2022
$4.0M
MOLECULAR CONTROL--SMOOTH MUSCLE CALPONIN EXPRESSIONR01HL062572 · NHLBI · UNIVERSITY OF ROCHESTER · PI MIANO, JOSEPH M · 1999 to 2011
$2.9M
Regulation of Vascular Smooth Muscle Cell Phenotype by a Novel Isoform of Glucose-6-Phosphate DehydrogenaseR01HL132574 · NHLBI · NEW YORK MEDICAL COLLEGE · PI GUPTE, SACHIN A, MIANO, JOSEPH M · 2017 to 2020
$2.5M
Molecular and Cellular Mechanisms of Calcific Aortic StenosisR01HL111770 · NHLBI · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI ZHOU, BIN · 2012 to 2015
$2.3M
Bifunctionality of Myocardin in MyogenesisR01HL091168 · NHLBI · UNIVERSITY OF ROCHESTER · PI MIANO, JOSEPH M · 2008 to 2011
$1.5M
Novel regulatory network involving non-coding role of an ASD candidate gene PTENR21MH099452 · NIMH · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI ZHENG, DEYOU · 2012 to 2013
$449k
NCI NIH HHS 5P01-CA013106NCI NIH HHS P01 CA013106NHLBI NIH HHS HL091168NHLBI NIH HHS HL111770NHLBI NIH HHS HL62572NHLBI NIH HHS R01 HL062572NHLBI NIH HHS R01 HL091168NHLBI NIH HHS R01 HL111770NHLBI NIH HHS R01 HL122686NHLBI NIH HHS R01 HL132574NIMH NIH HHS MH099452NIMH NIH HHS R21 MH099452
6 · The paper itself

Abstract

objectiveLong noncoding RNAs (lncRNAs) represent a rapidly growing class of RNA genes with functions related primarily to transcriptional and post-transcriptional control of gene expression. There is a paucity of information about lncRNA expression and function in human vascular cells. Thus, we set out to identify novel lncRNA genes in human vascular smooth muscle cells and to gain insight into their role in the control of smooth muscle cell phenotypes. APPROACH AND

resultsRNA sequencing (RNA-seq) of human coronary artery smooth muscle cells revealed 31 unannotated lncRNAs, including a vascular cell-enriched lncRNA (Smooth muscle and Endothelial cell-enriched migration/differentiation-associated long NonCoding RNA [SENCR]). Strand-specific reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends indicate that SENCR is transcribed antisense from the 5' end of the FLI1 gene and exists as 2 splice variants. RNA fluorescence in situ hybridization and biochemical fractionation studies demonstrate SENCR is a cytoplasmic lncRNA. Consistent with this observation, knockdown studies reveal little to no cis-acting effect of SENCR on FLI1 or neighboring gene expression. RNA-seq experiments in smooth muscle cells after SENCR knockdown disclose decreased expression of Myocardin and numerous smooth muscle contractile genes, whereas several promigratory genes are increased. Reverse transcription PCR and Western blotting experiments validate several differentially expressed genes after SENCR knockdown. Loss-of-function studies in scratch wound and Boyden chamber assays support SENCR as an inhibitor of smooth muscle cell migration.

conclusionsSENCR is a new vascular cell-enriched, cytoplasmic lncRNA that seems to stabilize the smooth muscle cell contractile phenotype.

Indexed as

Cells, CulturedHumansMuscle, Smooth, VascularMyocytes, Smooth MuscleProto-Oncogene Protein c-fli-1RNA, Long NoncodingRNA, MessengerVasoconstrictionFLI1 protein, humanProto-Oncogene Protein c-fli-1RNA, Long NoncodingRNA, Messengercell migrationendothelial cellsmyocytes, smooth muscleRNA, long noncodingRNA sequence

Identifiers

PMID24578380
PMCPMC4024079
OpenAlexW2152948086

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.