ArticleOrigins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life2014
Rapid RNA exchange in aqueous two-phase system and coacervate droplets.
Article in Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Competition Between Liquid-Liquid Crystalline Phase Separation (LLCPS) and Liquid-Liquid Phase Separation (LLPS) in Amyloid Fibril Colloidal Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stabilization of Polymer-Polyoxometalate Coacervate Droplets by Divalent Cations.ACS macro letters · 2026Article
- Emergent Chemical Reactivity and Complexity of RNA Condensates.Angewandte Chemie (International ed. in English) · 2025Article
- Nonenzymatic Primer Extension Experiments in the Presence of Coacervates.Current protocols · 2025Article
- Hybrid Protocells Based on Coacervate-Templated Fatty Acid Vesicles Combine Improved Membrane Stability with Functional Interior Protocytoplasm.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Did the exposure of coacervate droplets to rain make them the first stable protocells?Science advances · 2024Article
- Fundamental Aspects of Phase-Separated Biomolecular Condensates.Chemical reviews · 2024Review
- Tuning interfacial fluidity and colloidal stability of membranized coacervate protocells.Communications chemistry · 2024Article
- Adenosine Triphosphate Mediates Phase Separation of Disordered Basic Proteins by Bridging Intermolecular Interaction Networks.Journal of the American Chemical Society · 2024Article
- RNA folding studies inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life.Science advances · 2023Article
- ATP Mediates Phase Separation of Disordered Basic Proteins by Bridging Intermolecular Interaction Networks.bioRxiv : the preprint server for biology · 2023Article
- Multispecies autocatalytic RNA reaction networks in coacervates.Communications chemistry · 2023Article
- Biomolecular Liquid-Liquid Phase Separation for Biotechnology.Biotech (Basel (Switzerland)) · 2023Review
- A Physicochemical Consideration of Prebiotic Microenvironments for Self-Assembly and Prebiotic Chemistry.Life (Basel, Switzerland) · 2022Review
- Understanding Supramolecular Assembly of Supercharged Proteins.ACS central science · 2022Article
- Manipulation of coacervate droplets with an electric field.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Charge-density reduction promotes ribozyme activity in RNA-peptide coacervates via RNA fluidization and magnesium partitioning.Nature chemistry · 2022Article
- Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges.Frontiers in molecular biosciences · 2022Review
- Sequencing the origins of life.BBA advances · 2022Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compartmentalization in a prebiotic setting is an important aspect of early cell formation and is crucial for the development of an artificial protocell system that effectively couples genotype and phenotype. Aqueous two-phase systems (ATPSs) and complex coacervates are phase separation phenomena that lead to the selective partitioning of biomolecules and have recently been proposed as membrane-free protocell models. We show in this study through fluorescence recovery after photobleaching (FRAP) microscopy that despite the ability of such systems to effectively concentrate RNA, there is a high rate of RNA exchange between phases in dextran/polyethylene glycol ATPS and ATP/poly-L-lysine coacervate droplets. In contrast to fatty acid vesicles, these systems would not allow effective segregation and consequent evolution of RNA, thus rendering these systems ineffective as model protocells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.